BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0253100 Os03g0253100|AK119618
         (512 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0253100  Phosphomevalonate kinase Erg8 family protein        895   0.0  
Os03g0686800  Hypothetical protein                                107   2e-23

>Os03g0253100 Phosphomevalonate kinase Erg8 family protein
          Length = 512

 Score =  895 bits (2313), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 451/512 (88%), Positives = 451/512 (88%)

Query: 1   MEVVASAPGKVLVAGGYLVLERPNAGLVLSTSARFYAIVRPLRDELSHDSWAWAWTDVKV 60
           MEVVASAPGKVLVAGGYLVLERPNAGLVLSTSARFYAIVRPLRDELSHDSWAWAWTDVKV
Sbjct: 1   MEVVASAPGKVLVAGGYLVLERPNAGLVLSTSARFYAIVRPLRDELSHDSWAWAWTDVKV 60

Query: 61  TSPQLSREAXXXXXXXXXXXXXXXXRESANPFVEQAVQFSVAAAKVTVTDKEGKEALDKL 120
           TSPQLSREA                RESANPFVEQAVQFSVAAAKVTVTDKEGKEALDKL
Sbjct: 61  TSPQLSREATYKLSLKKSTLQLTSSRESANPFVEQAVQFSVAAAKVTVTDKEGKEALDKL 120

Query: 121 LLQGLHITILGCNDFYSYRKQIEARGLPLTPEVLLSLPPFCSITFNSEVANGTMTGENCK 180
           LLQGLHITILGCNDFYSYRKQIEARGLPLTPEVLLSLPPFCSITFNSEVANGTMTGENCK
Sbjct: 121 LLQGLHITILGCNDFYSYRKQIEARGLPLTPEVLLSLPPFCSITFNSEVANGTMTGENCK 180

Query: 181 PEVAKTGLGXXXXXXXXXXXXLLHYLGTVNLSCLGQSSGGNAAGRDLDLLHAIAQSAHCI 240
           PEVAKTGLG            LLHYLGTVNLSCLGQSSGGNAAGRDLDLLHAIAQSAHCI
Sbjct: 181 PEVAKTGLGSSAAMTTSVVAALLHYLGTVNLSCLGQSSGGNAAGRDLDLLHAIAQSAHCI 240

Query: 241 AQGKIGSGFDVSAAVYGSQRYTRFSPEILSSAQVTGGHCMADVVADIVTQSWDHEKVKFS 300
           AQGKIGSGFDVSAAVYGSQRYTRFSPEILSSAQVTGGHCMADVVADIVTQSWDHEKVKFS
Sbjct: 241 AQGKIGSGFDVSAAVYGSQRYTRFSPEILSSAQVTGGHCMADVVADIVTQSWDHEKVKFS 300

Query: 301 LPPLMXXXXXXXXXXXXXXXXXXXXXKQWQKSDPQKSKETWSKLGIANSVLENQLRNLNK 360
           LPPLM                     KQWQKSDPQKSKETWSKLGIANSVLENQLRNLNK
Sbjct: 301 LPPLMSLLLGEPGTGGSSTPSMVGSVKQWQKSDPQKSKETWSKLGIANSVLENQLRNLNK 360

Query: 361 LAEDHWEAYESVLRSCSRLTCSKWTEVATNQHQELIVRSLLAARDAFLEIRLHMREMGIA 420
           LAEDHWEAYESVLRSCSRLTCSKWTEVATNQHQELIVRSLLAARDAFLEIRLHMREMGIA
Sbjct: 361 LAEDHWEAYESVLRSCSRLTCSKWTEVATNQHQELIVRSLLAARDAFLEIRLHMREMGIA 420

Query: 421 AGVPIEPESQTQLLDATMNMEXXXXXXXXXXXXFDAVFSVILGEASDAVAKAWSSAGVLP 480
           AGVPIEPESQTQLLDATMNME            FDAVFSVILGEASDAVAKAWSSAGVLP
Sbjct: 421 AGVPIEPESQTQLLDATMNMEGVLLAGVPGAGGFDAVFSVILGEASDAVAKAWSSAGVLP 480

Query: 481 LLVREDPRGVSLEAGDPRTREVSTAVSSIQIN 512
           LLVREDPRGVSLEAGDPRTREVSTAVSSIQIN
Sbjct: 481 LLVREDPRGVSLEAGDPRTREVSTAVSSIQIN 512



>Os03g0686800 Hypothetical protein
          Length = 268

 Score =  107 bits (268), Expect = 2e-23,   Method: Compositional matrix adjust.
 Identities = 59/92 (64%), Positives = 64/92 (69%), Gaps = 13/92 (14%)

Query: 5   ASAPGKVLVAGGYLVLERPNAGLVLSTSARFYAIVRPLRDELSHD-SWAWAWTDVKVTSP 63
           ASAPGKVL        ERPNAGLVL T+ARFYA+V P RD+L+HD SWAWAWT+VKVTSP
Sbjct: 60  ASAPGKVL--------ERPNAGLVLITTARFYAVVLPPRDQLTHDESWAWAWTNVKVTSP 111

Query: 64  QLSREAXXXXXXXXXXXXXXXXRESANPFVEQ 95
           QLSREA                RESANPFVEQ
Sbjct: 112 QLSREA----TYKLSPKKSTSSRESANPFVEQ 139



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.130    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,190,686
Number of extensions: 538720
Number of successful extensions: 1165
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1161
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 407
Effective length of database: 11,553,331
Effective search space: 4702205717
Effective search space used: 4702205717
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 158 (65.5 bits)