BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0251000 Os03g0251000|AK104668
         (158 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0251000  Plant lipid transfer/seed storage/trypsin-alph...   157   2e-39
Os03g0250801  Plant lipid transfer/seed storage/trypsin-alph...   101   2e-22

>Os03g0251000 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
           domain containing protein
          Length = 158

 Score =  157 bits (398), Expect = 2e-39,   Method: Compositional matrix adjust.
 Identities = 90/158 (56%), Positives = 90/158 (56%)

Query: 1   MASKQAMNXXXXXXXXXXXXXXXXNGQLNPCQXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MASKQAMN                NGQLNPCQ                            
Sbjct: 1   MASKQAMNSALFSALVVVVLASAVNGQLNPCQAPAPTPTGTPAPAPAPVVTPTPAPTPVP 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXKCPLALINLNACISVGLGNPLLNQACCSQLSSLPSD 120
                                   KCPLALINLNACISVGLGNPLLNQACCSQLSSLPSD
Sbjct: 61  TPSPAPTPSPTVAPVNPPSPTPPPKCPLALINLNACISVGLGNPLLNQACCSQLSSLPSD 120

Query: 121 TAAVCLCEAIKVNALVNLKVKIPDILKVCGKVSAVVCV 158
           TAAVCLCEAIKVNALVNLKVKIPDILKVCGKVSAVVCV
Sbjct: 121 TAAVCLCEAIKVNALVNLKVKIPDILKVCGKVSAVVCV 158



>Os03g0250801 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
           domain containing protein
          Length = 148

 Score =  101 bits (252), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 52/67 (77%), Positives = 57/67 (85%)

Query: 92  NLNACISVGLGNPLLNQACCSQLSSLPSDTAAVCLCEAIKVNALVNLKVKIPDILKVCGK 151
           +L  C++V LGN LLNQ CCSQLSSLPSDTAA CLCEAIKV ALVNLKV +P+ILK CGK
Sbjct: 81  DLGVCLNVALGNQLLNQQCCSQLSSLPSDTAAFCLCEAIKVKALVNLKVNVPNILKACGK 140

Query: 152 VSAVVCV 158
           VSAV CV
Sbjct: 141 VSAVTCV 147



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.133    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,502,399
Number of extensions: 64532
Number of successful extensions: 232
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 233
Number of HSP's successfully gapped: 2
Length of query: 158
Length of database: 17,035,801
Length adjustment: 93
Effective length of query: 65
Effective length of database: 12,179,899
Effective search space: 791693435
Effective search space used: 791693435
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 152 (63.2 bits)