BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0250000 Os03g0250000|AK121051
         (143 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0250000  EF-Hand type domain containing protein              238   1e-63
Os10g0177200  EF-Hand type domain containing protein              118   1e-27

>Os03g0250000 EF-Hand type domain containing protein
          Length = 143

 Score =  238 bits (607), Expect = 1e-63,   Method: Compositional matrix adjust.
 Identities = 121/143 (84%), Positives = 121/143 (84%)

Query: 1   MERTRSWFXXXXXXXXXXXXXXXXXXXXXXQKVVVDGSEIRELVEDREAFGMFAESKFRE 60
           MERTRSWF                      QKVVVDGSEIRELVEDREAFGMFAESKFRE
Sbjct: 1   MERTRSWFGRRRRRKARGEGGGGGGEEGEGQKVVVDGSEIRELVEDREAFGMFAESKFRE 60

Query: 61  LDADGDGRLSVRELQPAVEGIGAALGLPARGSSPNADHIYSEVQNAGKFFVVLPGEKKNR 120
           LDADGDGRLSVRELQPAVEGIGAALGLPARGSSPNADHIYSEVQNAGKFFVVLPGEKKNR
Sbjct: 61  LDADGDGRLSVRELQPAVEGIGAALGLPARGSSPNADHIYSEVQNAGKFFVVLPGEKKNR 120

Query: 121 SECCAGQPPDTSFEKNRLVLLSR 143
           SECCAGQPPDTSFEKNRLVLLSR
Sbjct: 121 SECCAGQPPDTSFEKNRLVLLSR 143



>Os10g0177200 EF-Hand type domain containing protein
          Length = 386

 Score =  118 bits (296), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 59/74 (79%), Positives = 64/74 (86%)

Query: 32  KVVVDGSEIRELVEDREAFGMFAESKFRELDADGDGRLSVRELQPAVEGIGAALGLPARG 91
           +VVVDGSEI +LVE++EAFG F E KFR LDADGDGRLSVRELQPAV  IGAA+GLPARG
Sbjct: 24  EVVVDGSEILQLVENKEAFGKFVEQKFRLLDADGDGRLSVRELQPAVADIGAAIGLPARG 83

Query: 92  SSPNADHIYSEVQN 105
           SS  ADHIYSEV N
Sbjct: 84  SSAQADHIYSEVLN 97



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,423,811
Number of extensions: 180417
Number of successful extensions: 613
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 612
Number of HSP's successfully gapped: 2
Length of query: 143
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 52
Effective length of database: 12,284,327
Effective search space: 638785004
Effective search space used: 638785004
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 151 (62.8 bits)