BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0242200 Os03g0242200|AK071529
         (338 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0242200  Ras GTPase family protein                           568   e-162
Os10g0135200  Ras GTPase family protein                           439   e-123

>Os03g0242200 Ras GTPase family protein
          Length = 338

 Score =  568 bits (1464), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 285/338 (84%), Positives = 285/338 (84%)

Query: 1   MFWKDXXXXXXXXXXXLEQNGVGPFGQVRVLVVGDSGVGKSSLVHLILKGSAIARPAQTV 60
           MFWKD           LEQNGVGPFGQVRVLVVGDSGVGKSSLVHLILKGSAIARPAQTV
Sbjct: 1   MFWKDSGGRSSGGGGGLEQNGVGPFGQVRVLVVGDSGVGKSSLVHLILKGSAIARPAQTV 60

Query: 61  GCAVGVKHITYXXXXXXXXXXXXDVQRNFFVELWDVSGHERYRTCRSIFYTQINGVIFVY 120
           GCAVGVKHITY            DVQRNFFVELWDVSGHERYRTCRSIFYTQINGVIFVY
Sbjct: 61  GCAVGVKHITYGSAGGSSNNIISDVQRNFFVELWDVSGHERYRTCRSIFYTQINGVIFVY 120

Query: 121 DLSQRKTKTNLNKWAVEVAETGTFSAXXXXXXXXXXXXXXXVIANKVDLVPRDGSRVSSG 180
           DLSQRKTKTNLNKWAVEVAETGTFSA               VIANKVDLVPRDGSRVSSG
Sbjct: 121 DLSQRKTKTNLNKWAVEVAETGTFSAPLGSGGPGGLPVPYLVIANKVDLVPRDGSRVSSG 180

Query: 181 SLVDFARQWVEKQGLLPSSEELPLTDSFPGNSGLLSAAKEARYDKEAVIKFFRMLIRRRF 240
           SLVDFARQWVEKQGLLPSSEELPLTDSFPGNSGLLSAAKEARYDKEAVIKFFRMLIRRRF
Sbjct: 181 SLVDFARQWVEKQGLLPSSEELPLTDSFPGNSGLLSAAKEARYDKEAVIKFFRMLIRRRF 240

Query: 241 FSNEPAAPSPWSLTPREDSILPVETLKDEVDSFQRKSGEDFMYKGVNPLPAQRNLASPPD 300
           FSNEPAAPSPWSLTPREDSILPVETLKDEVDSFQRKSGEDFMYKGVNPLPAQRNLASPPD
Sbjct: 241 FSNEPAAPSPWSLTPREDSILPVETLKDEVDSFQRKSGEDFMYKGVNPLPAQRNLASPPD 300

Query: 301 LSPQQPVFSLDNYRYHRYXXXXXXXXXXXXXXXEDIDV 338
           LSPQQPVFSLDNYRYHRY               EDIDV
Sbjct: 301 LSPQQPVFSLDNYRYHRYSSPSLPDVSSSRTSREDIDV 338



>Os10g0135200 Ras GTPase family protein
          Length = 343

 Score =  439 bits (1129), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 224/321 (69%), Positives = 246/321 (76%), Gaps = 7/321 (2%)

Query: 24  PFGQVRVLVVGDSGVGKSSLVHLILKGSAIARPAQTVGCAVGVKHITYXXXXXXXXXXXX 83
           P GQVRVLVVGDSGVGKSSLVHLILKGSAIARP QT+GCAV VKHITY            
Sbjct: 24  PCGQVRVLVVGDSGVGKSSLVHLILKGSAIARPPQTIGCAVDVKHITYGSPGSSSNSINS 83

Query: 84  ---DVQRNFFVELWDVSGHERYRTCRSIFYTQINGVIFVYDLSQRKTKTNLNKWAVEVAE 140
              D +RNFFVELWDVSGHERY+ CRS+FY+QINGVIFVYDLSQRKTKTNLNKWAVEVAE
Sbjct: 84  IKGDAERNFFVELWDVSGHERYKECRSLFYSQINGVIFVYDLSQRKTKTNLNKWAVEVAE 143

Query: 141 TGTFSAXXXXXXXXXXXXXXXVIANKVDLVPRDGSRVSSGSLVDFARQWVEKQGLLPSSE 200
           +GTFSA               VIANKVD+ PRDG RVSSG+LVD ARQWVEKQGLLPSSE
Sbjct: 144 SGTFSAPLGSGGPGGLPVPYLVIANKVDIAPRDGKRVSSGNLVDVARQWVEKQGLLPSSE 203

Query: 201 ELPLTDSFPGNSGLLSAAKEARYDKEAVIKFFRMLIRRRFFSNEPAAPSPWSLTPREDSI 260
           ELPL +SFPGNSGLL+AAK ARYDKEA++KFFRMLIRRR+FSNE  APSPWSLTPRED+I
Sbjct: 204 ELPLAESFPGNSGLLTAAKVARYDKEALVKFFRMLIRRRYFSNELPAPSPWSLTPREDTI 263

Query: 261 LPVETLKDEVDSFQRKS--GEDFMYKGVNPLPAQRNLASPPDLSPQQPV-FSLDNYRYHR 317
           LPVET  D+ D FQRKS  G+ + Y GV PLPAQRNL  PP L PQQP+  S +NYRYHR
Sbjct: 264 LPVETTNDD-DLFQRKSYAGQSYKYSGVTPLPAQRNLTPPPTLYPQQPMSSSSENYRYHR 322

Query: 318 YXXXXXXXXXXXXXXXEDIDV 338
           +                DI++
Sbjct: 323 FSSSAIPDASSSRTNRADINI 343



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.136    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,060,607
Number of extensions: 396309
Number of successful extensions: 1367
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1384
Number of HSP's successfully gapped: 3
Length of query: 338
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 237
Effective length of database: 11,762,187
Effective search space: 2787638319
Effective search space used: 2787638319
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)