BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0231800 Os03g0231800|AK065032
         (530 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0231800  Similar to Squalene monooxygenase 1 (EC 1.14.9...   941   0.0  
Os03g0231700  Similar to Squalene monooxygenase 2 (EC 1.14.9...   871   0.0  

>Os03g0231800 Similar to Squalene monooxygenase 1 (EC 1.14.99.7)
          Length = 530

 Score =  941 bits (2433), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 471/530 (88%), Positives = 471/530 (88%)

Query: 1   MAEVAAGTGQLIGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MAEVAAGTGQLIGV                                              
Sbjct: 1   MAEVAAGTGQLIGVAVATLLAAIFLAAALLGSRRRRRRAPLAGKPAAVGGCGVADGEGCG 60

Query: 61  XXXRTDVIXXXXXXXXXXLAYTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELG 120
              RTDVI          LAYTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELG
Sbjct: 61  GDGRTDVIVVGAGVAGSALAYTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELG 120

Query: 121 LEDCVQEIDAQRVLGYALFKDGKDTKLSYPLEKFHSDVAGRSFHNGRFIQRMRQKAASLP 180
           LEDCVQEIDAQRVLGYALFKDGKDTKLSYPLEKFHSDVAGRSFHNGRFIQRMRQKAASLP
Sbjct: 121 LEDCVQEIDAQRVLGYALFKDGKDTKLSYPLEKFHSDVAGRSFHNGRFIQRMRQKAASLP 180

Query: 181 NVQLEQGTVTSLVEEDGTVKGVKYKTKSGEELKAYAPLTIVCDGCFSNLRRALCSPKVDV 240
           NVQLEQGTVTSLVEEDGTVKGVKYKTKSGEELKAYAPLTIVCDGCFSNLRRALCSPKVDV
Sbjct: 181 NVQLEQGTVTSLVEEDGTVKGVKYKTKSGEELKAYAPLTIVCDGCFSNLRRALCSPKVDV 240

Query: 241 PSCFVGLVLENCQLPHANHGHVVLANPSPILFYPISSTEVRCLVDVPGQKVPSIANGEMA 300
           PSCFVGLVLENCQLPHANHGHVVLANPSPILFYPISSTEVRCLVDVPGQKVPSIANGEMA
Sbjct: 241 PSCFVGLVLENCQLPHANHGHVVLANPSPILFYPISSTEVRCLVDVPGQKVPSIANGEMA 300

Query: 301 KYLKTVVAPQIPPEIYDSFIAAIDKGSIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
           KYLKTVVAPQIPPEIYDSFIAAIDKGSIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT
Sbjct: 301 KYLKTVVAPQIPPEIYDSFIAAIDKGSIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360

Query: 361 GGGMTVALSDIVVLRNLLKPLRNLHDASALCKYLESFYTLRKPVASTINTLAGALYKVFS 420
           GGGMTVALSDIVVLRNLLKPLRNLHDASALCKYLESFYTLRKPVASTINTLAGALYKVFS
Sbjct: 361 GGGMTVALSDIVVLRNLLKPLRNLHDASALCKYLESFYTLRKPVASTINTLAGALYKVFS 420

Query: 421 ASPDQARNEMRQACFDYLSLGGVFSNGPIALLSGLNPRPLSLVAHFFAVAIYGVGRLMLP 480
           ASPDQARNEMRQACFDYLSLGGVFSNGPIALLSGLNPRPLSLVAHFFAVAIYGVGRLMLP
Sbjct: 421 ASPDQARNEMRQACFDYLSLGGVFSNGPIALLSGLNPRPLSLVAHFFAVAIYGVGRLMLP 480

Query: 481 LPSPKRMWIGVRLISSACGIIFPIIKAEGVRHMFFPATVPAYYRAPRPME 530
           LPSPKRMWIGVRLISSACGIIFPIIKAEGVRHMFFPATVPAYYRAPRPME
Sbjct: 481 LPSPKRMWIGVRLISSACGIIFPIIKAEGVRHMFFPATVPAYYRAPRPME 530



>Os03g0231700 Similar to Squalene monooxygenase 2 (EC 1.14.99.7)
          Length = 524

 Score =  871 bits (2250), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 419/467 (89%), Positives = 440/467 (94%)

Query: 64  RTDVIXXXXXXXXXXLAYTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 123
           RTD++          LAYTLGKDGRRVHVIERD+TEPDRIVGELLQPGGYLKL+ELGLED
Sbjct: 56  RTDIVIVGAGVAGSALAYTLGKDGRRVHVIERDMTEPDRIVGELLQPGGYLKLMELGLED 115

Query: 124 CVQEIDAQRVLGYALFKDGKDTKLSYPLEKFHSDVAGRSFHNGRFIQRMRQKAASLPNVQ 183
           CV+EIDAQRVLGYAL KDG++TKLSYPLEKFHSDVAGRSFHNGRFIQ+MRQKAASLPNV 
Sbjct: 116 CVEEIDAQRVLGYALLKDGRNTKLSYPLEKFHSDVAGRSFHNGRFIQKMRQKAASLPNVH 175

Query: 184 LEQGTVTSLVEEDGTVKGVKYKTKSGEELKAYAPLTIVCDGCFSNLRRALCSPKVDVPSC 243
           LEQGTVTSL+EE GTVKGV+YKTKSGEELKAYAPLTIVCDGCFSNLRR LCSPKVDVPSC
Sbjct: 176 LEQGTVTSLLEEGGTVKGVQYKTKSGEELKAYAPLTIVCDGCFSNLRRVLCSPKVDVPSC 235

Query: 244 FVGLVLENCQLPHANHGHVVLANPSPILFYPISSTEVRCLVDVPGQKVPSIANGEMAKYL 303
           FVGLVLENCQLPH NHGHV+LANPSPIL YPISSTE+RCLVD+PGQKVPS+A GEMAKYL
Sbjct: 236 FVGLVLENCQLPHPNHGHVILANPSPILCYPISSTEIRCLVDIPGQKVPSMATGEMAKYL 295

Query: 304 KTVVAPQIPPEIYDSFIAAIDKGSIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGG 363
           KTVVAPQIPPE++DSFIAAIDKGSIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTGGG
Sbjct: 296 KTVVAPQIPPELHDSFIAAIDKGSIRTMPNRSMPAAPLPTPGALLMGDAFNMRHPLTGGG 355

Query: 364 MTVALSDIVVLRNLLKPLRNLHDASALCKYLESFYTLRKPVASTINTLAGALYKVFSASP 423
           MTVA SDIVVLRNLLKPL NLHDA +LCKYLESFYTLRKPVASTINTLAGALYKVF AS 
Sbjct: 356 MTVAFSDIVVLRNLLKPLGNLHDAPSLCKYLESFYTLRKPVASTINTLAGALYKVFCAST 415

Query: 424 DQARNEMRQACFDYLSLGGVFSNGPIALLSGLNPRPLSLVAHFFAVAIYGVGRLMLPLPS 483
           DQA+NEMR+ACFDYLSLGGVFSNGPIALLSGLNPRPLSLVAHFFAVAIYGVGRLMLP+PS
Sbjct: 416 DQAKNEMREACFDYLSLGGVFSNGPIALLSGLNPRPLSLVAHFFAVAIYGVGRLMLPVPS 475

Query: 484 PKRMWIGVRLISSACGIIFPIIKAEGVRHMFFPATVPAYYRAPRPME 530
           PKRMWIG RL+S ACGIIFPIIKAEGVR MFFPATVPAYYRAP PME
Sbjct: 476 PKRMWIGARLVSGACGIIFPIIKAEGVRQMFFPATVPAYYRAPPPME 522



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.139    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 17,278,109
Number of extensions: 765501
Number of successful extensions: 1484
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1484
Number of HSP's successfully gapped: 2
Length of query: 530
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 424
Effective length of database: 11,501,117
Effective search space: 4876473608
Effective search space used: 4876473608
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 158 (65.5 bits)