BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0227500 Os03g0227500|AK063077
         (106 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0227500  Hypothetical protein                                167   2e-42
Os03g0669600  Hypothetical protein                                 65   8e-12

>Os03g0227500 Hypothetical protein
          Length = 106

 Score =  167 bits (422), Expect = 2e-42,   Method: Compositional matrix adjust.
 Identities = 83/83 (100%), Positives = 83/83 (100%)

Query: 1  MITRAKLVEQLREHQIRSAQSYSAALAVFSPSPHIASRRDLKVALFYAILFCFVMVSCYV 60
          MITRAKLVEQLREHQIRSAQSYSAALAVFSPSPHIASRRDLKVALFYAILFCFVMVSCYV
Sbjct: 1  MITRAKLVEQLREHQIRSAQSYSAALAVFSPSPHIASRRDLKVALFYAILFCFVMVSCYV 60

Query: 61 ALYLRWFRLSAIFVVFGILLPVG 83
          ALYLRWFRLSAIFVVFGILLPVG
Sbjct: 61 ALYLRWFRLSAIFVVFGILLPVG 83



>Os03g0669600 Hypothetical protein
          Length = 89

 Score = 65.5 bits (158), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 36/83 (43%), Positives = 50/83 (60%), Gaps = 17/83 (20%)

Query: 1  MITRAKLVEQLREHQIRSAQSYSAALAVFSPSPHIASRRDLKVALFYAILFCFVMVSCYV 60
          MITR+KLVEQLR++QIRS                   +RD+  A+ + +L CF+++S Y+
Sbjct: 1  MITRSKLVEQLRDYQIRSQH-----------------KRDIIGAVSWGLLCCFLIISSYM 43

Query: 61 ALYLRWFRLSAIFVVFGILLPVG 83
           LY R F LSA+ +  GILLP G
Sbjct: 44 TLYFRHFWLSAVIISLGILLPAG 66



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.336    0.141    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,187,740
Number of extensions: 60778
Number of successful extensions: 342
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 341
Number of HSP's successfully gapped: 2
Length of query: 106
Length of database: 17,035,801
Length adjustment: 74
Effective length of query: 32
Effective length of database: 13,171,965
Effective search space: 421502880
Effective search space used: 421502880
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 149 (62.0 bits)