BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0175800 Os03g0175800|AK109502
(310 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0175800 Conserved hypothetical protein 335 4e-92
Os10g0397600 71 9e-13
>Os03g0175800 Conserved hypothetical protein
Length = 310
Score = 335 bits (858), Expect = 4e-92, Method: Compositional matrix adjust.
Identities = 198/310 (63%), Positives = 198/310 (63%)
Query: 1 MERWAAPKVTAGSARRYVADQPSFSSTLLDAIYKSMDEQPXXXXXXXXXXXXXXXXXXXX 60
MERWAAPKVTAGSARRYVADQPSFSSTLLDAIYKSMDEQP
Sbjct: 1 MERWAAPKVTAGSARRYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGVEAVAAAAKKQH 60
Query: 61 XXXLHYGNYYKPSLAGSYRARAPGPHATTXXXXXXXXXXXXXXXXXXXXHHRRLRPIRTT 120
LHYGNYYKPSLAGSYRARAPGPHATT HHRRLRPIRTT
Sbjct: 61 EAALHYGNYYKPSLAGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTT 120
Query: 121 VXXXXXXXXXXXXXXXXXXSIRAKLRDLRKPASPGARLAGFLNSIFAGKRXXXXXXXXXX 180
V SIRAKLRDLRKPASPGARLAGFLNSIFAGKR
Sbjct: 121 VPGGAPGPAPEKKAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAGKRAPATPPSATA 180
Query: 181 XXXXXXXXXXXXXXXXXXKTPSTRGQAKRTVRFLDSDTESLASSTVVDRRRVPVEAVQQM 240
KTPSTRGQAKRTVRFLDSDTESLASSTVVDRRRVPVEAVQQM
Sbjct: 181 GAESACSTASSYSRSCLSKTPSTRGQAKRTVRFLDSDTESLASSTVVDRRRVPVEAVQQM 240
Query: 241 LLQRXXXXXXXXXXXXXXXXXXXXXXXNFAAIAPAGAAYRDELPVYETTRVALNRAIGHG 300
LLQR NFAAIAPAGAAYRDELPVYETTRVALNRAIGHG
Sbjct: 241 LLQRMEMESDEDDDESSDASSDLFELENFAAIAPAGAAYRDELPVYETTRVALNRAIGHG 300
Query: 301 YGHGRSARVV 310
YGHGRSARVV
Sbjct: 301 YGHGRSARVV 310
>Os10g0397600
Length = 337
Score = 71.2 bits (173), Expect = 9e-13, Method: Compositional matrix adjust.
Identities = 60/161 (37%), Positives = 64/161 (39%), Gaps = 17/161 (10%)
Query: 20 DQPSFSSTLLDAIYKSMDEQPXXXXXXXXXXXXXXXXXXXXXXXLHYGNYYKPSLAGSYR 79
DQPSFSSTLLDAI SMDE HY YKPSLA SYR
Sbjct: 27 DQPSFSSTLLDAICDSMDEG-GEDGRTRNAASAAAKKRQEAANSYHYYYCYKPSLAASYR 85
Query: 80 ARAPGPHATTXXXXXXXXXXXXXXXXXXXXHHRRLRPIRTTVXXXXXX----------XX 129
A AP +T RRLRPIRT+
Sbjct: 86 A-APALGSTADCPGRGYFSSSEVEYSL-----RRLRPIRTSAAGGAGDGAAVARKQRHEQ 139
Query: 130 XXXXXXXXXXSIRAKLRDLRKPASPGARLAGFLNSIFAGKR 170
A R R+PASPGARLA LNSIF+GKR
Sbjct: 140 PDVEKTAKTKPGSASARACRRPASPGARLASLLNSIFSGKR 180
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.131 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,647,188
Number of extensions: 157277
Number of successful extensions: 308
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 307
Number of HSP's successfully gapped: 2
Length of query: 310
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 209
Effective length of database: 11,762,187
Effective search space: 2458297083
Effective search space used: 2458297083
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)