BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0172400 Os03g0172400|Os03g0172400
         (131 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0172400  Proteinase inhibitor I25A and I25B, type 2 and...   222   5e-59
Os03g0172300                                                       99   9e-22
Os03g0172600                                                       92   1e-19

>Os03g0172400 Proteinase inhibitor I25A and I25B, type 2 and phytocystatins
           domain containing protein
          Length = 131

 Score =  222 bits (566), Expect = 5e-59,   Method: Compositional matrix adjust.
 Identities = 114/131 (87%), Positives = 114/131 (87%)

Query: 1   MRPSSLSTVVLSVIFTAALLAVAKADGGSXXXXXXXXXXXXXXXXXNVNDKSIQQVGQSA 60
           MRPSSLSTVVLSVIFTAALLAVAKADGGS                 NVNDKSIQQVGQSA
Sbjct: 1   MRPSSLSTVVLSVIFTAALLAVAKADGGSVTAAAAAPPPAAWTAVANVNDKSIQQVGQSA 60

Query: 61  VRIYGLSTNKTYLRFVNVVSGQTQPCNGGYNYRLLVTVAGPGATTARYDALMWGILGTTN 120
           VRIYGLSTNKTYLRFVNVVSGQTQPCNGGYNYRLLVTVAGPGATTARYDALMWGILGTTN
Sbjct: 61  VRIYGLSTNKTYLRFVNVVSGQTQPCNGGYNYRLLVTVAGPGATTARYDALMWGILGTTN 120

Query: 121 WKLLSFTLAAN 131
           WKLLSFTLAAN
Sbjct: 121 WKLLSFTLAAN 131



>Os03g0172300 
          Length = 135

 Score = 99.0 bits (245), Expect = 9e-22,   Method: Compositional matrix adjust.
 Identities = 54/83 (65%), Positives = 60/83 (72%), Gaps = 2/83 (2%)

Query: 47  NVNDKSIQQVGQSAVRIYGLSTNKTYLRFVNVVSGQTQPCNGGYNYRLLVTV-AGPGATT 105
           NVNDKSIQQ GQ A+ IY   T   +LR+VNVVSGQTQP NGGYNYRL+VTV  GP   T
Sbjct: 49  NVNDKSIQQAGQFALWIYRQITRLYFLRYVNVVSGQTQPYNGGYNYRLVVTVYGGPNWKT 108

Query: 106 ARYDALMWGILG-TTNWKLLSFT 127
             YDA +WGI G TT+W   SFT
Sbjct: 109 TLYDADVWGIPGTTTHWWFRSFT 131



>Os03g0172600 
          Length = 209

 Score = 92.0 bits (227), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 50/82 (60%), Positives = 61/82 (74%), Gaps = 3/82 (3%)

Query: 47  NVNDKSIQQVGQSAVRIYGLSTNKTYLRFVNVVSGQTQPCNGG-YNYRLLVTVAGPGATT 105
           NVND +IQQVG+ AV+ Y L+T    L +VNVVSGQTQPC+GG  NY+L++ VA  G  T
Sbjct: 127 NVNDYTIQQVGKFAVQSYCLNTGAK-LVYVNVVSGQTQPCSGGGSNYQLVINVAA-GVRT 184

Query: 106 ARYDALMWGILGTTNWKLLSFT 127
           A+Y   +WGILGTT WKL SFT
Sbjct: 185 AQYSVFVWGILGTTTWKLWSFT 206



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.131    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,526,701
Number of extensions: 104783
Number of successful extensions: 177
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 178
Number of HSP's successfully gapped: 3
Length of query: 131
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 42
Effective length of database: 12,388,755
Effective search space: 520327710
Effective search space used: 520327710
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 150 (62.4 bits)