BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0129000 Os03g0129000|AK073895
         (218 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0129000  Phospho-ethanolamine N-methyltransferase famil...   421   e-118
Os10g0200800  Phospho-ethanolamine N-methyltransferase famil...   205   2e-53

>Os03g0129000 Phospho-ethanolamine N-methyltransferase family protein
          Length = 218

 Score =  421 bits (1082), Expect = e-118,   Method: Compositional matrix adjust.
 Identities = 209/218 (95%), Positives = 209/218 (95%)

Query: 1   MGDEATQXXXXXXXXXHALCFAGIVAAHQLSGRGMLVSNPAYALRLLVVFEAPLVIAVFS 60
           MGDEATQ         HALCFAGIVAAHQLSGRGMLVSNPAYALRLLVVFEAPLVIAVFS
Sbjct: 1   MGDEATQVSASSAVAVHALCFAGIVAAHQLSGRGMLVSNPAYALRLLVVFEAPLVIAVFS 60

Query: 61  LLRRNPKRCSFLKAAARGLLGLPIGAFLNAFGAIVLGAPIGIKYWAATTYWSLLMSLFTF 120
           LLRRNPKRCSFLKAAARGLLGLPIGAFLNAFGAIVLGAPIGIKYWAATTYWSLLMSLFTF
Sbjct: 61  LLRRNPKRCSFLKAAARGLLGLPIGAFLNAFGAIVLGAPIGIKYWAATTYWSLLMSLFTF 120

Query: 121 VPAACVFGASKVNWQAVLSHSIYCGSTDSVDYMISAPAHGAVIGAWLGAWPMPLDWERPW 180
           VPAACVFGASKVNWQAVLSHSIYCGSTDSVDYMISAPAHGAVIGAWLGAWPMPLDWERPW
Sbjct: 121 VPAACVFGASKVNWQAVLSHSIYCGSTDSVDYMISAPAHGAVIGAWLGAWPMPLDWERPW 180

Query: 181 QEWPISVTYGSVAGHLIGMAISLALVVTHKRRGRAKAD 218
           QEWPISVTYGSVAGHLIGMAISLALVVTHKRRGRAKAD
Sbjct: 181 QEWPISVTYGSVAGHLIGMAISLALVVTHKRRGRAKAD 218



>Os10g0200800 Phospho-ethanolamine N-methyltransferase family protein
          Length = 344

 Score =  205 bits (521), Expect = 2e-53,   Method: Compositional matrix adjust.
 Identities = 109/151 (72%), Positives = 116/151 (76%), Gaps = 17/151 (11%)

Query: 68  RCSFLKAAARGLLGLPIGAFLNAFGAIVLGAPIGIKYWAATTYWSLLMSLFTFVPAACVF 127
              F  A ++  LG   GAFLNAFGAIVLGAPIGIKYWAATTYWSLLMSLFT      V 
Sbjct: 211 NVQFAPAKSQAPLG---GAFLNAFGAIVLGAPIGIKYWAATTYWSLLMSLFT-----VVL 262

Query: 128 GASKVNWQAVLSHSIYCGSTDSVDYMISAPAHGAVIGAWLGAWPMPLDWERPWQEWPISV 187
             S + W A+   ++        DYMISAPAHGAVIGAWLGAWPMPLDWERPWQEWPISV
Sbjct: 263 NVSAI-WTAIFGAAM--------DYMISAPAHGAVIGAWLGAWPMPLDWERPWQEWPISV 313

Query: 188 TYGSVAGHLIGMAISLALVVTHKRRGRAKAD 218
           TYGSVAGHLIGMAISLAL+V HKRRGRAKAD
Sbjct: 314 TYGSVAGHLIGMAISLALIVAHKRRGRAKAD 344



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.137    0.449 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,399,750
Number of extensions: 306706
Number of successful extensions: 960
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 959
Number of HSP's successfully gapped: 2
Length of query: 218
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 121
Effective length of database: 11,971,043
Effective search space: 1448496203
Effective search space used: 1448496203
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 154 (63.9 bits)