BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0112400 Os03g0112400|AK063082
         (153 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0112400  AP2 clathrin adaptor, alpha and beta chain, ap...   277   2e-75

>Os03g0112400 AP2 clathrin adaptor, alpha and beta chain, appendage domain
           containing protein
          Length = 153

 Score =  277 bits (708), Expect = 2e-75,   Method: Compositional matrix adjust.
 Identities = 137/153 (89%), Positives = 137/153 (89%)

Query: 1   QPITLSPEEFFPQWKALNVQNLKVQEVVKGVKPLPLPEIASLFMSLHLAVAPGLDNNPNN 60
           QPITLSPEEFFPQWKALNVQNLKVQEVVKGVKPLPLPEIASLFMSLHLAVAPGLDNNPNN
Sbjct: 1   QPITLSPEEFFPQWKALNVQNLKVQEVVKGVKPLPLPEIASLFMSLHLAVAPGLDNNPNN 60

Query: 61  LVACTTFFSETTRAMLCLVRIETDPQDRTQLRLTVASGDQYLTFELKEYIKEHLIDIPRV 120
           LVACTTFFSETTRAMLCLVRIETDPQDRTQLRLTVASGDQYLTFELKEYIKEHLIDIPRV
Sbjct: 61  LVACTTFFSETTRAMLCLVRIETDPQDRTQLRLTVASGDQYLTFELKEYIKEHLIDIPRV 120

Query: 121 QTASXXXXXXXXXXXXXXXXTYNDPGAMLAGLL 153
           QTAS                TYNDPGAMLAGLL
Sbjct: 121 QTASPPVPVQPQLPPAAVPATYNDPGAMLAGLL 153



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,452,059
Number of extensions: 147770
Number of successful extensions: 260
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 259
Number of HSP's successfully gapped: 1
Length of query: 153
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 61
Effective length of database: 12,232,113
Effective search space: 746158893
Effective search space used: 746158893
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)