BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0754700 Os02g0754700|AK066904
         (487 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0754700  Similar to Histidyl-tRNA synthetase (EC 6.1.1.21)   900   0.0  
Os05g0150900  Histidyl-tRNA synthetase (EC 6.1.1.21) (Histid...   152   5e-37

>Os02g0754700 Similar to Histidyl-tRNA synthetase (EC 6.1.1.21)
          Length = 487

 Score =  900 bits (2325), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 449/460 (97%), Positives = 449/460 (97%)

Query: 28  TFRTSSLPRRHQRLFASAPSSSTVENGEPSSPVEPSDGASVVDVNPPRGTRDFPPEDMRL 87
           TFRTSSLPRRHQRLFASAPSSSTVENGEPSSPVEPSDGASVVDVNPPRGTRDFPPEDMRL
Sbjct: 28  TFRTSSLPRRHQRLFASAPSSSTVENGEPSSPVEPSDGASVVDVNPPRGTRDFPPEDMRL 87

Query: 88  RTWLFDQFREVSRVMAFEEVDFPVLESEALFIRKAGEEITQQLYNFEDKGGRRVVLRPEI 147
           RTWLFDQFREVSRVMAFEEVDFPVLESEALFIRKAGEEITQQLYNFEDKGGRRVVLRPEI
Sbjct: 88  RTWLFDQFREVSRVMAFEEVDFPVLESEALFIRKAGEEITQQLYNFEDKGGRRVVLRPEI 147

Query: 148 TPSLARLVIKQGKSVSLPLKWFTIGQCWXXXXXXXXXXXEHYQWNMDIFGMPKVRAESEL 207
           TPSLARLVIKQGKSVSLPLKWFTIGQCW           EHYQWNMDIFGMPKVRAESEL
Sbjct: 148 TPSLARLVIKQGKSVSLPLKWFTIGQCWRYERMTRGRRREHYQWNMDIFGMPKVRAESEL 207

Query: 208 LQAIILLFERLGITSSDVGIRVSSRKVLQAVLNMYSIPEHLFTEVCVIVDKLGKLTREEI 267
           LQAIILLFERLGITSSDVGIRVSSRKVLQAVLNMYSIPEHLFTEVCVIVDKLGKLTREEI
Sbjct: 208 LQAIILLFERLGITSSDVGIRVSSRKVLQAVLNMYSIPEHLFTEVCVIVDKLGKLTREEI 267

Query: 268 EKKLVTIGLSSEAVLGIIEVLSLKSLPKLEEVLGSDVEAVADLKKLFSFAEQYGYADWIC 327
           EKKLVTIGLSSEAVLGIIEVLSLKSLPKLEEVLGSDVEAVADLKKLFSFAEQYGYADWIC
Sbjct: 268 EKKLVTIGLSSEAVLGIIEVLSLKSLPKLEEVLGSDVEAVADLKKLFSFAEQYGYADWIC 327

Query: 328 FDASVVRGLAYYTGIVFEAFDRDGKLRAICGGGRYDRLLSTFGSEDIPACGFGFGDAVIV 387
           FDASVVRGLAYYTGIVFEAFDRDGKLRAICGGGRYDRLLSTFGSEDIPACGFGFGDAVIV
Sbjct: 328 FDASVVRGLAYYTGIVFEAFDRDGKLRAICGGGRYDRLLSTFGSEDIPACGFGFGDAVIV 387

Query: 388 ELLKEKGLLPDLSRQIDDIVFPLDEELEGPACSVASSLRRQGRSVDLVEDKRLKWVFKHA 447
           ELLKEKGLLPDLSRQIDDIVFPLDEELEGPACSVASSLRRQGRSVDLVEDKRLKWVFKHA
Sbjct: 388 ELLKEKGLLPDLSRQIDDIVFPLDEELEGPACSVASSLRRQGRSVDLVEDKRLKWVFKHA 447

Query: 448 ERINAGRLILVGKSEWERGMVRVKILSTREEFEVKAGELQ 487
           ERINAGRLILVGKSEWERGMVRVKILSTREEFEVKAGELQ
Sbjct: 448 ERINAGRLILVGKSEWERGMVRVKILSTREEFEVKAGELQ 487



>Os05g0150900 Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase)
           (HisRS)
          Length = 788

 Score =  152 bits (384), Expect = 5e-37,   Method: Compositional matrix adjust.
 Identities = 121/425 (28%), Positives = 210/425 (49%), Gaps = 25/425 (5%)

Query: 74  PRGTRDFPPEDMRLRTWLFDQFREVSRVMAFEEVDFPVLESEALFIRKAGEEITQQLYNF 133
           P+GTRDF  E M +R   F     V ++     +D PV E     + K GE+ ++ +Y+ 
Sbjct: 356 PKGTRDFGKEQMAIREHAFSIITGVFKMHGAVSLDTPVFELRETLMGKYGED-SKLIYDL 414

Query: 134 EDKGGRRVVLRPEITPSLARLVIKQGKSVSLPLKWFTIGQCWXXXXXXXXXXXEHYQWNM 193
            D+GG    LR ++T   AR V     S    LK + I + +           E YQ + 
Sbjct: 415 ADQGGELCSLRYDLTVPFARYVAMNNIS---SLKRYQIAKVYRRDNPSKGRYREFYQCDF 471

Query: 194 DIFGM-PKVRAESELLQAIILLFERLGITSSDVGIRVSSRKVLQAVLNMYSIPEHLFTEV 252
           DI G+   +  + E+++ +  L ++L I + +  I+++ RK+L  +L +  +P   F  V
Sbjct: 472 DIAGVYETMEPDFEVIKVLTELLDQLDIGTYE--IKLNHRKLLDGMLEICGVPPEKFRTV 529

Query: 253 CVIVDKLGKLTREEIEKKLVT-IGLSSEAVLGIIEVLSLKSLPKLEEVL----------- 300
           C  +DKL K T E+++K+LV   G+S+E    I +++  +  P LE +L           
Sbjct: 530 CSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLVKTRG-PPLEVLLELRKEGSKFMG 588

Query: 301 -GSDVEAVADLKKLFSFAEQYGYADWICFDASVVRGLAYYTGIVFEA-FDRDGKLRAICG 358
               V A+ +L+ LF   ++      I FD S+ RGL YYTG+++EA F    ++ +I  
Sbjct: 589 NAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLDYYTGVIYEAVFKGTTQVGSIAA 648

Query: 359 GGRYDRLLSTFGSEDIPACGFGFGDAVIVELLKEKGLLPDLSRQIDD--IVFPLDEELEG 416
           GGRYD L+  F  + +PA G   G   +  +++++ +  +  R  +   +V  + ++L  
Sbjct: 649 GGRYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEMEKNQIRATETEVLVSIIGKDLI- 707

Query: 417 PACSVASSLRRQGRSVDLVEDKRLKWVFKHAERINAGRLILVGKSEWERGMVRVKILSTR 476
            A  + S L   G   +     R++   K+A +     ++LVG+SE   G V++K L+  
Sbjct: 708 LAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPWMVLVGESEISSGKVKLKNLAAS 767

Query: 477 EEFEV 481
           +E EV
Sbjct: 768 QEEEV 772



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,347,547
Number of extensions: 670922
Number of successful extensions: 1872
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1868
Number of HSP's successfully gapped: 2
Length of query: 487
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 382
Effective length of database: 11,553,331
Effective search space: 4413372442
Effective search space used: 4413372442
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)