BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0628800 Os02g0628800|AK058725
(73 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0628800 Similar to Ubiquitin-like protein 5 148 7e-37
Os12g0143150 Ubiquitin domain containing protein 143 3e-35
>Os02g0628800 Similar to Ubiquitin-like protein 5
Length = 73
Score = 148 bits (374), Expect = 7e-37, Method: Compositional matrix adjust.
Identities = 73/73 (100%), Positives = 73/73 (100%)
Query: 1 MIEVVLNDRLGKKVRVKCNEDDTIGDLKKLVAAQTGTRAEKIRIQKWYNIYKDHITLADY 60
MIEVVLNDRLGKKVRVKCNEDDTIGDLKKLVAAQTGTRAEKIRIQKWYNIYKDHITLADY
Sbjct: 1 MIEVVLNDRLGKKVRVKCNEDDTIGDLKKLVAAQTGTRAEKIRIQKWYNIYKDHITLADY 60
Query: 61 EIHDGMGLELYYD 73
EIHDGMGLELYYD
Sbjct: 61 EIHDGMGLELYYD 73
>Os12g0143150 Ubiquitin domain containing protein
Length = 73
Score = 143 bits (361), Expect = 3e-35, Method: Compositional matrix adjust.
Identities = 70/73 (95%), Positives = 71/73 (97%)
Query: 1 MIEVVLNDRLGKKVRVKCNEDDTIGDLKKLVAAQTGTRAEKIRIQKWYNIYKDHITLADY 60
MIEVVLNDRLGKKVRVKCNEDDTIGDLKKLVAAQTGTR EKIRIQKWYNIYKDHITL DY
Sbjct: 1 MIEVVLNDRLGKKVRVKCNEDDTIGDLKKLVAAQTGTRPEKIRIQKWYNIYKDHITLRDY 60
Query: 61 EIHDGMGLELYYD 73
EIHDGMGLELYY+
Sbjct: 61 EIHDGMGLELYYN 73
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.320 0.140 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,330,110
Number of extensions: 68065
Number of successful extensions: 171
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 171
Number of HSP's successfully gapped: 2
Length of query: 73
Length of database: 17,035,801
Length adjustment: 44
Effective length of query: 29
Effective length of database: 14,738,385
Effective search space: 427413165
Effective search space used: 427413165
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)