BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0565200 Os02g0565200|AK102047
         (191 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0565200  Microsomal signal peptidase 25 kDa subunit fam...   360   e-100
Os04g0446300  Microsomal signal peptidase 25 kDa subunit fam...   261   3e-70

>Os02g0565200 Microsomal signal peptidase 25 kDa subunit family protein
          Length = 191

 Score =  360 bits (923), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 177/191 (92%), Positives = 177/191 (92%)

Query: 1   MATDGGGATPSPAAKKANLLDPYSIKHLLDETVSEVAKSKGYPEDARLSNRRXXXXXXXX 60
           MATDGGGATPSPAAKKANLLDPYSIKHLLDETVSEVAKSKGYPEDARLSNRR        
Sbjct: 1   MATDGGGATPSPAAKKANLLDPYSIKHLLDETVSEVAKSKGYPEDARLSNRRLAVGAAVI 60

Query: 61  XXXXXXQFYPRKFPENRDVLLACIALYAALNVALQIVTYTKEKNAILFTYPPAGSFNRTG 120
                 QFYPRKFPENRDVLLACIALYAALNVALQIVTYTKEKNAILFTYPPAGSFNRTG
Sbjct: 61  AVALLAQFYPRKFPENRDVLLACIALYAALNVALQIVTYTKEKNAILFTYPPAGSFNRTG 120

Query: 121 LVISSKLPRLSDMYTLTIASADPHSKSSSEPVHFTKSVTKWFTKDGVLVEGLFWKDVEKL 180
           LVISSKLPRLSDMYTLTIASADPHSKSSSEPVHFTKSVTKWFTKDGVLVEGLFWKDVEKL
Sbjct: 121 LVISSKLPRLSDMYTLTIASADPHSKSSSEPVHFTKSVTKWFTKDGVLVEGLFWKDVEKL 180

Query: 181 IDDYNSDHRSK 191
           IDDYNSDHRSK
Sbjct: 181 IDDYNSDHRSK 191



>Os04g0446300 Microsomal signal peptidase 25 kDa subunit family protein
          Length = 192

 Score =  261 bits (666), Expect = 3e-70,   Method: Compositional matrix adjust.
 Identities = 133/192 (69%), Positives = 157/192 (81%), Gaps = 1/192 (0%)

Query: 1   MATDGGGATPSPAA-KKANLLDPYSIKHLLDETVSEVAKSKGYPEDARLSNRRXXXXXXX 59
           MA+DG  ATP+ A+ KKANLLDP+SIKHLLDET+S+V KSKGY ED RL N +       
Sbjct: 1   MASDGAAATPAKASPKKANLLDPHSIKHLLDETISDVVKSKGYAEDTRLGNWKLAIGAAV 60

Query: 60  XXXXXXXQFYPRKFPENRDVLLACIALYAALNVALQIVTYTKEKNAILFTYPPAGSFNRT 119
                  QFYP+KFP+NR+ LL CIALY  LNV L I++YTKEK+AI+FT+PPAGSFN T
Sbjct: 61  IAIALLAQFYPKKFPQNREFLLGCIALYVVLNVVLLILSYTKEKDAIMFTHPPAGSFNST 120

Query: 120 GLVISSKLPRLSDMYTLTIASADPHSKSSSEPVHFTKSVTKWFTKDGVLVEGLFWKDVEK 179
           GLVISSKLPR SDMYT+TIASADP S S+++PVHFTKSVTKWFTK+GVLVEGLFWKDVE+
Sbjct: 121 GLVISSKLPRFSDMYTITIASADPQSISANKPVHFTKSVTKWFTKEGVLVEGLFWKDVER 180

Query: 180 LIDDYNSDHRSK 191
           LIDDYN++ + K
Sbjct: 181 LIDDYNTERKGK 192



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.131    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,264,022
Number of extensions: 211699
Number of successful extensions: 581
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 580
Number of HSP's successfully gapped: 2
Length of query: 191
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 96
Effective length of database: 12,075,471
Effective search space: 1159245216
Effective search space used: 1159245216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 153 (63.5 bits)