BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0214300 Os02g0214300|Os02g0214300
         (302 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0214300  Similar to Nuclear ribonuclease Z (EC 3.1.26.1...   570   e-163
Os09g0482680  Similar to Ribonuclease Z, chloroplast precurs...   347   9e-96

>Os02g0214300 Similar to Nuclear ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNase
           Z) (tRNA 3 endonuclease) (Zinc phosphodiesterase ELAC)
           (Fragment)
          Length = 302

 Score =  570 bits (1468), Expect = e-163,   Method: Compositional matrix adjust.
 Identities = 280/302 (92%), Positives = 280/302 (92%)

Query: 1   MANSGKXXXXXXXXXXXXXXXXXXXXXXLTVEGYPVEGISIGGQETCVIFPTLSAAFDIG 60
           MANSGK                      LTVEGYPVEGISIGGQETCVIFPTLSAAFDIG
Sbjct: 1   MANSGKSSPAATSTTAPPPGRPKAKAPPLTVEGYPVEGISIGGQETCVIFPTLSAAFDIG 60

Query: 61  RCPQRAVSQEFLFISHAHLDHIGGLPMYVATRGLYRQRPPTIFIPACLRDPVERLFELHR 120
           RCPQRAVSQEFLFISHAHLDHIGGLPMYVATRGLYRQRPPTIFIPACLRDPVERLFELHR
Sbjct: 61  RCPQRAVSQEFLFISHAHLDHIGGLPMYVATRGLYRQRPPTIFIPACLRDPVERLFELHR 120

Query: 121 SMDQSELSHNLVPLEIGQEHELRRDLKVKAFKTYHAIPSQGYVIYTVKQKLKPEYLGLPG 180
           SMDQSELSHNLVPLEIGQEHELRRDLKVKAFKTYHAIPSQGYVIYTVKQKLKPEYLGLPG
Sbjct: 121 SMDQSELSHNLVPLEIGQEHELRRDLKVKAFKTYHAIPSQGYVIYTVKQKLKPEYLGLPG 180

Query: 181 SEIKQLKLSGVEITNTLTVPEIAFTGDTMADFILDPDNADVLKAKILVVESTFVDDSVTI 240
           SEIKQLKLSGVEITNTLTVPEIAFTGDTMADFILDPDNADVLKAKILVVESTFVDDSVTI
Sbjct: 181 SEIKQLKLSGVEITNTLTVPEIAFTGDTMADFILDPDNADVLKAKILVVESTFVDDSVTI 240

Query: 241 EHAREYGHTHLFEILNQCDKLENKAILLIHFSARYTAEEIDIAINKLPPSFRSRVHALKE 300
           EHAREYGHTHLFEILNQCDKLENKAILLIHFSARYTAEEIDIAINKLPPSFRSRVHALKE
Sbjct: 241 EHAREYGHTHLFEILNQCDKLENKAILLIHFSARYTAEEIDIAINKLPPSFRSRVHALKE 300

Query: 301 GF 302
           GF
Sbjct: 301 GF 302



>Os09g0482680 Similar to Ribonuclease Z, chloroplast precursor (EC 3.1.26.11)
           (RNase Z) (tRNase Z) (tRNA 3 endonuclease) (Zinc
           phosphodiesterase CPZ)
          Length = 365

 Score =  347 bits (889), Expect = 9e-96,   Method: Compositional matrix adjust.
 Identities = 156/272 (57%), Positives = 208/272 (76%)

Query: 31  VEGYPVEGISIGGQETCVIFPTLSAAFDIGRCPQRAVSQEFLFISHAHLDHIGGLPMYVA 90
           VEGY +EG+S+GG ETCV  P+L+ AFDIGR P  AVSQ++LFI+HAHLDHIGGLPMY+A
Sbjct: 89  VEGYAIEGVSVGGHETCVTVPSLNVAFDIGRGPLFAVSQDYLFITHAHLDHIGGLPMYIA 148

Query: 91  TRGLYRQRPPTIFIPACLRDPVERLFELHRSMDQSELSHNLVPLEIGQEHELRRDLKVKA 150
           TRGLY  +PP +F+P C++D VE L ++HR M Q +L   LV L++G+  E+R DL  + 
Sbjct: 149 TRGLYNLKPPVVFVPPCIKDDVEDLLQIHRRMSQVDLKVELVALDLGETFEIRNDLVARP 208

Query: 151 FKTYHAIPSQGYVIYTVKQKLKPEYLGLPGSEIKQLKLSGVEITNTLTVPEIAFTGDTMA 210
           F+T+HAIPSQGYVIY+V++KLK +Y  L G++I ++K SG EIT+T+  PE+AFTGDT +
Sbjct: 209 FETHHAIPSQGYVIYSVRRKLKKQYAHLKGNQIMKMKQSGAEITDTILYPEVAFTGDTKS 268

Query: 211 DFILDPDNADVLKAKILVVESTFVDDSVTIEHAREYGHTHLFEILNQCDKLENKAILLIH 270
           DFILDP NAD L+AK+L+ E+TF+DD + ++HARE+GH HL EI+       NKAI+L H
Sbjct: 269 DFILDPRNADALRAKVLITEATFLDDQIDVDHAREHGHMHLSEIMEHSQWFRNKAIVLTH 328

Query: 271 FSARYTAEEIDIAINKLPPSFRSRVHALKEGF 302
           FS RY+ E+I  A++KL     S+V AL EGF
Sbjct: 329 FSNRYSLEDIRQAVSKLQSKLSSKVVALTEGF 360



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.139    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,331,589
Number of extensions: 372990
Number of successful extensions: 630
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 630
Number of HSP's successfully gapped: 2
Length of query: 302
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 202
Effective length of database: 11,814,401
Effective search space: 2386509002
Effective search space used: 2386509002
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 156 (64.7 bits)