BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0978000 Os01g0978000|AK103874
         (134 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0978000  Similar to Uncoupling protein                       204   2e-53
Os11g0707800  Uncoupling protein                                  110   2e-25

>Os01g0978000 Similar to Uncoupling protein
          Length = 134

 Score =  204 bits (518), Expect = 2e-53,   Method: Compositional matrix adjust.
 Identities = 101/115 (87%), Positives = 101/115 (87%)

Query: 20  EVCTIPLDTAKVRLQLQKKAALAXXXXXXXXXXXXXXIMCIAREEGVAALWNGIIPGLHR 79
           EVCTIPLDTAKVRLQLQKKAALA              IMCIAREEGVAALWNGIIPGLHR
Sbjct: 20  EVCTIPLDTAKVRLQLQKKAALATGGGGGTTGGMLGTIMCIAREEGVAALWNGIIPGLHR 79

Query: 80  QCVYGGLRIALYEPVKAFFIRDGDTVAGGVSLFAKILAALMTANQFTSKFHVLRT 134
           QCVYGGLRIALYEPVKAFFIRDGDTVAGGVSLFAKILAALMTANQFTSKFHVLRT
Sbjct: 80  QCVYGGLRIALYEPVKAFFIRDGDTVAGGVSLFAKILAALMTANQFTSKFHVLRT 134



>Os11g0707800 Uncoupling protein
          Length = 301

 Score =  110 bits (276), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 59/102 (57%), Positives = 64/102 (62%), Gaps = 5/102 (4%)

Query: 20  EVCTIPLDTAKVRLQLQKKAALAXXXXXXXXXXXXXXIMCIAREEGVAALWNGIIPGLHR 79
           EVCTIPLDTAKVRLQLQK  A                   IAREEG AALW GI+PGLHR
Sbjct: 27  EVCTIPLDTAKVRLQLQKNVA---ADAAPKYRGLLGTAATIAREEGAAALWKGIVPGLHR 83

Query: 80  QCVYGGLRIALYEPVKAFFIRDGDTVAGGVSLFAKILAALMT 121
           QC+YGGLRI LYEPVK+F++  G    G V L  KI A   T
Sbjct: 84  QCIYGGLRIGLYEPVKSFYV--GKDHVGDVPLTKKIAAGFTT 123



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.140    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,099,311
Number of extensions: 96985
Number of successful extensions: 295
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 295
Number of HSP's successfully gapped: 2
Length of query: 134
Length of database: 17,035,801
Length adjustment: 90
Effective length of query: 44
Effective length of database: 12,336,541
Effective search space: 542807804
Effective search space used: 542807804
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 150 (62.4 bits)