BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0914300 Os01g0914300|AK105463
         (103 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0914300  Plant lipid transfer/seed storage/trypsin-alph...   159   4e-40
Os01g0914100  Plant lipid transfer/seed storage/trypsin-alph...    85   1e-17
>Os01g0914300 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
           domain containing protein
          Length = 103

 Score =  159 bits (402), Expect = 4e-40,   Method: Compositional matrix adjust.
 Identities = 83/103 (80%), Positives = 83/103 (80%)

Query: 1   MAGKVSXXXXXXXXXXXXXXXXXXXXSMCGVDRSAVALCRSYCTVGSAEKAPTKECCKAV 60
           MAGKVS                    SMCGVDRSAVALCRSYCTVGSAEKAPTKECCKAV
Sbjct: 1   MAGKVSAVVLVALVVVAAAAGGAAGLSMCGVDRSAVALCRSYCTVGSAEKAPTKECCKAV 60

Query: 61  ANADFQCLCDRRDMLRNLENIDADRATQIPSKCGVPGASSSCK 103
           ANADFQCLCDRRDMLRNLENIDADRATQIPSKCGVPGASSSCK
Sbjct: 61  ANADFQCLCDRRDMLRNLENIDADRATQIPSKCGVPGASSSCK 103
>Os01g0914100 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
           domain containing protein
          Length = 111

 Score = 85.1 bits (209), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 45/76 (59%), Positives = 52/76 (68%), Gaps = 1/76 (1%)

Query: 28  MCGVDRSAVAL-CRSYCTVGSAEKAPTKECCKAVANADFQCLCDRRDMLRNLENIDADRA 86
           +CGVD   +A  C SYC  GS E+AP +ECC AV  ADF+CLC  RD LR + NIDA RA
Sbjct: 36  VCGVDADRMAADCGSYCRAGSRERAPRRECCDAVRGADFKCLCKYRDELRVMGNIDAARA 95

Query: 87  TQIPSKCGVPGASSSC 102
            QIPSKC + GA  SC
Sbjct: 96  MQIPSKCRIKGAPKSC 111
  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.129    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,781,880
Number of extensions: 83851
Number of successful extensions: 226
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 228
Number of HSP's successfully gapped: 2
Length of query: 103
Length of database: 17,035,801
Length adjustment: 71
Effective length of query: 32
Effective length of database: 13,328,607
Effective search space: 426515424
Effective search space used: 426515424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)