BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0914300 Os01g0914300|AK105463
(103 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0914300 Plant lipid transfer/seed storage/trypsin-alph... 159 4e-40
Os01g0914100 Plant lipid transfer/seed storage/trypsin-alph... 85 1e-17
>Os01g0914300 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
domain containing protein
Length = 103
Score = 159 bits (402), Expect = 4e-40, Method: Compositional matrix adjust.
Identities = 83/103 (80%), Positives = 83/103 (80%)
Query: 1 MAGKVSXXXXXXXXXXXXXXXXXXXXSMCGVDRSAVALCRSYCTVGSAEKAPTKECCKAV 60
MAGKVS SMCGVDRSAVALCRSYCTVGSAEKAPTKECCKAV
Sbjct: 1 MAGKVSAVVLVALVVVAAAAGGAAGLSMCGVDRSAVALCRSYCTVGSAEKAPTKECCKAV 60
Query: 61 ANADFQCLCDRRDMLRNLENIDADRATQIPSKCGVPGASSSCK 103
ANADFQCLCDRRDMLRNLENIDADRATQIPSKCGVPGASSSCK
Sbjct: 61 ANADFQCLCDRRDMLRNLENIDADRATQIPSKCGVPGASSSCK 103
>Os01g0914100 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
domain containing protein
Length = 111
Score = 85.1 bits (209), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 45/76 (59%), Positives = 52/76 (68%), Gaps = 1/76 (1%)
Query: 28 MCGVDRSAVAL-CRSYCTVGSAEKAPTKECCKAVANADFQCLCDRRDMLRNLENIDADRA 86
+CGVD +A C SYC GS E+AP +ECC AV ADF+CLC RD LR + NIDA RA
Sbjct: 36 VCGVDADRMAADCGSYCRAGSRERAPRRECCDAVRGADFKCLCKYRDELRVMGNIDAARA 95
Query: 87 TQIPSKCGVPGASSSC 102
QIPSKC + GA SC
Sbjct: 96 MQIPSKCRIKGAPKSC 111
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.320 0.129 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,781,880
Number of extensions: 83851
Number of successful extensions: 226
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 228
Number of HSP's successfully gapped: 2
Length of query: 103
Length of database: 17,035,801
Length adjustment: 71
Effective length of query: 32
Effective length of database: 13,328,607
Effective search space: 426515424
Effective search space used: 426515424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)