BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0899700 Os01g0899700|AK110796
         (412 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0899700  Pollen Ole e 1 allergen and extensin domain co...   346   2e-95

>Os01g0899700 Pollen Ole e 1 allergen and extensin domain containing protein
          Length = 412

 Score =  346 bits (887), Expect = 2e-95,   Method: Compositional matrix adjust.
 Identities = 165/178 (92%), Positives = 165/178 (92%)

Query: 1   MKFSMRGQPKGIPWLVLAMLIATFAMLTEGAXXXXXXXXXXXXXYAPVIKVIGKVYCYRC 60
           MKFSMRGQPKGIPWLVLAMLIATFAMLTEGA             YAPVIKVIGKVYCYRC
Sbjct: 1   MKFSMRGQPKGIPWLVLAMLIATFAMLTEGATSLSPPSLSLTPTYAPVIKVIGKVYCYRC 60

Query: 61  FNEAHPEESHGKEHLKGAMVKVTCQANDQALVGFGYTQDNGKYSVSITGLPLSSTYGADS 120
           FNEAHPEESHGKEHLKGAMVKVTCQANDQALVGFGYTQDNGKYSVSITGLPLSSTYGADS
Sbjct: 61  FNEAHPEESHGKEHLKGAMVKVTCQANDQALVGFGYTQDNGKYSVSITGLPLSSTYGADS 120

Query: 121 CKVELHSAPGGSDCNVPIELNLSGLSVYSKSNEEVVLQANQVMAFASQKTFGFCSKPH 178
           CKVELHSAPGGSDCNVPIELNLSGLSVYSKSNEEVVLQANQVMAFASQKTFGFCSKPH
Sbjct: 121 CKVELHSAPGGSDCNVPIELNLSGLSVYSKSNEEVVLQANQVMAFASQKTFGFCSKPH 178




 Score =  137 bits (344), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 80/123 (65%), Positives = 80/123 (65%)

Query: 290 QHSPPANSYVSXXXXXXXXXXXXXXXXXXXXXXXXXXANHYSPPPYNFGSSPPTYQYSXX 349
           QHSPPANSYVS                          ANHYSPPPYNFGSSPPTYQYS  
Sbjct: 290 QHSPPANSYVSPPLAHQYPPPPYKSPPIPPYYFNSPPANHYSPPPYNFGSSPPTYQYSPP 349

Query: 350 XXXXXXXXXXXXXXXEMSPPAHATSPQPLVHYSPPPPLQHAGISSTTPSVNSYQSPPPVN 409
                          EMSPPAHATSPQPLVHYSPPPPLQHAGISSTTPSVNSYQSPPPVN
Sbjct: 350 LLPKTPKYLPPKVPLEMSPPAHATSPQPLVHYSPPPPLQHAGISSTTPSVNSYQSPPPVN 409

Query: 410 QLS 412
           QLS
Sbjct: 410 QLS 412



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.129    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,300,456
Number of extensions: 399507
Number of successful extensions: 2370
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 2361
Number of HSP's successfully gapped: 2
Length of query: 412
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 309
Effective length of database: 11,657,759
Effective search space: 3602247531
Effective search space used: 3602247531
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 157 (65.1 bits)