BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0851700 Os01g0851700|AK103367
         (841 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0851700  Similar to Cytosolic starch phosphorylase (Fra...  1666   0.0  
Os03g0758100  Similar to Plastidic alpha 1,4-glucan phosphor...   569   e-162

>Os01g0851700 Similar to Cytosolic starch phosphorylase (Fragment)
          Length = 841

 Score = 1666 bits (4314), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 804/841 (95%), Positives = 804/841 (95%)

Query: 1   MPESNGAACGAAEKVKXXXXXXXXXXXXIAGNISFHAQYSPHFSPLAFGPEQAFYSTAES 60
           MPESNGAACGAAEKVK            IAGNISFHAQYSPHFSPLAFGPEQAFYSTAES
Sbjct: 1   MPESNGAACGAAEKVKPAASPASEEPAAIAGNISFHAQYSPHFSPLAFGPEQAFYSTAES 60

Query: 61  VRDHLVQRWNETYLHFHKTDPKQTYYLSMEYLQGRALTNAVGNLGITGAYAEAVKKFGYE 120
           VRDHLVQRWNETYLHFHKTDPKQTYYLSMEYLQGRALTNAVGNLGITGAYAEAVKKFGYE
Sbjct: 61  VRDHLVQRWNETYLHFHKTDPKQTYYLSMEYLQGRALTNAVGNLGITGAYAEAVKKFGYE 120

Query: 121 LEALVGQEKDXXXXXXXXXXXXSCFLDSMATLNLPAWGYGLRYRYGLFKQCITKEGQEEI 180
           LEALVGQEKD            SCFLDSMATLNLPAWGYGLRYRYGLFKQCITKEGQEEI
Sbjct: 121 LEALVGQEKDAALGNGGLGRLASCFLDSMATLNLPAWGYGLRYRYGLFKQCITKEGQEEI 180

Query: 181 AEDWLEKFSPWEIVRHDIVYPIRFFGHVEILPDGSRKWVGGEVLNALAYDVPIPGYKTKN 240
           AEDWLEKFSPWEIVRHDIVYPIRFFGHVEILPDGSRKWVGGEVLNALAYDVPIPGYKTKN
Sbjct: 181 AEDWLEKFSPWEIVRHDIVYPIRFFGHVEILPDGSRKWVGGEVLNALAYDVPIPGYKTKN 240

Query: 241 AISLRLWDAKASAEDFNLFQFNDGQYESAAQLHARAQQICAVLYPGDATEEGKLLRLKQQ 300
           AISLRLWDAKASAEDFNLFQFNDGQYESAAQLHARAQQICAVLYPGDATEEGKLLRLKQQ
Sbjct: 241 AISLRLWDAKASAEDFNLFQFNDGQYESAAQLHARAQQICAVLYPGDATEEGKLLRLKQQ 300

Query: 301 YFLCSASLQDIFFRFKERKADRVSGKWSEFPAKVAVQLNDTHPTLAIPELMRLLMDVEGL 360
           YFLCSASLQDIFFRFKERKADRVSGKWSEFPAKVAVQLNDTHPTLAIPELMRLLMDVEGL
Sbjct: 301 YFLCSASLQDIFFRFKERKADRVSGKWSEFPAKVAVQLNDTHPTLAIPELMRLLMDVEGL 360

Query: 361 GWDEAWDITNKTIAYTNHTVLPEALEKWSQIVMRKLLPRHMEIIEEIDKRFKEMVISTRK 420
           GWDEAWDITNKTIAYTNHTVLPEALEKWSQIVMRKLLPRHMEIIEEIDKRFKEMVISTRK
Sbjct: 361 GWDEAWDITNKTIAYTNHTVLPEALEKWSQIVMRKLLPRHMEIIEEIDKRFKEMVISTRK 420

Query: 421 EMEGKIDSMRILDNSNPQKPVVRMANLCVVSAHTVNGVAELHSNILKEELFADYLSIWPN 480
           EMEGKIDSMRILDNSNPQKPVVRMANLCVVSAHTVNGVAELHSNILKEELFADYLSIWPN
Sbjct: 421 EMEGKIDSMRILDNSNPQKPVVRMANLCVVSAHTVNGVAELHSNILKEELFADYLSIWPN 480

Query: 481 KFQNKTNGITPRRWLRFCNPELSEIVTKWLKTDQWTSNLDLLTGLRKFADDEKLHAEWXX 540
           KFQNKTNGITPRRWLRFCNPELSEIVTKWLKTDQWTSNLDLLTGLRKFADDEKLHAEW  
Sbjct: 481 KFQNKTNGITPRRWLRFCNPELSEIVTKWLKTDQWTSNLDLLTGLRKFADDEKLHAEWAS 540

Query: 541 XXXXXXXXXXXHVLDVTGVTIDPNSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKGMSAE 600
                      HVLDVTGVTIDPNSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKGMSAE
Sbjct: 541 AKLASKKRLAKHVLDVTGVTIDPNSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKGMSAE 600

Query: 601 ERQKVTPRTVMIGGKAFATYTNAKRIVKLVNDVGAVVNNDPDVNKYLKVVFIPNYNVSVA 660
           ERQKVTPRTVMIGGKAFATYTNAKRIVKLVNDVGAVVNNDPDVNKYLKVVFIPNYNVSVA
Sbjct: 601 ERQKVTPRTVMIGGKAFATYTNAKRIVKLVNDVGAVVNNDPDVNKYLKVVFIPNYNVSVA 660

Query: 661 EVLIPGSELSQHISTAGMEASGTSNMKFSLNGCVIIGTLDGANVEIREEVGQENFFLFGA 720
           EVLIPGSELSQHISTAGMEASGTSNMKFSLNGCVIIGTLDGANVEIREEVGQENFFLFGA
Sbjct: 661 EVLIPGSELSQHISTAGMEASGTSNMKFSLNGCVIIGTLDGANVEIREEVGQENFFLFGA 720

Query: 721 KADQVAGLRKDRENGLFKPDPRFEEAKQLIRSGAFGTYDYAPLLDSLEGNSGFGRGDYFL 780
           KADQVAGLRKDRENGLFKPDPRFEEAKQLIRSGAFGTYDYAPLLDSLEGNSGFGRGDYFL
Sbjct: 721 KADQVAGLRKDRENGLFKPDPRFEEAKQLIRSGAFGTYDYAPLLDSLEGNSGFGRGDYFL 780

Query: 781 VGYDFPSYIDAQAQVDEAYKDKKKWIKMSILNTAGSGKFSSDRTIAQYAKEIWGITASPV 840
           VGYDFPSYIDAQAQVDEAYKDKKKWIKMSILNTAGSGKFSSDRTIAQYAKEIWGITASPV
Sbjct: 781 VGYDFPSYIDAQAQVDEAYKDKKKWIKMSILNTAGSGKFSSDRTIAQYAKEIWGITASPV 840

Query: 841 S 841
           S
Sbjct: 841 S 841



>Os03g0758100 Similar to Plastidic alpha 1,4-glucan phosphorylase 2 (EC 2.4.1.1)
           (Fragment)
          Length = 629

 Score =  569 bits (1466), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 265/417 (63%), Positives = 323/417 (77%), Gaps = 2/417 (0%)

Query: 421 EMEGKIDSMRILDNSNPQKP-VVRMANLCVVSAHTVNGVAELHSNILKEELFADYLSIWP 479
           E E   D +     S+P+ P VVRMANLCVV  H+VNGVA +HS I+KE++F  +  +WP
Sbjct: 207 ENEDSEDELDPFVKSDPKLPRVVRMANLCVVGGHSVNGVAAIHSEIVKEDVFNSFYEMWP 266

Query: 480 NKFQNKTNGITPRRWLRFCNPELSEIVTKWLKTDQWTSNLDLLTGLRKFADDEKLHAEWX 539
            KFQNKTNG+TPRRW+RFCNPELS I++KW+ +D W  N D L  L+KFADDE L +EW 
Sbjct: 267 AKFQNKTNGVTPRRWIRFCNPELSAIISKWIGSDDWVLNTDKLAELKKFADDEDLQSEWR 326

Query: 540 XXXXXXXXXXXXHVLDVTGVTIDPNSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKGMSA 599
                        + + TG  + P+++FD+Q+KRIHEYKRQLLNILG VYRYKK+K MSA
Sbjct: 327 AAKKANKVKVVSLIREKTGYIVSPDAMFDVQVKRIHEYKRQLLNILGIVYRYKKMKEMSA 386

Query: 600 EER-QKVTPRTVMIGGKAFATYTNAKRIVKLVNDVGAVVNNDPDVNKYLKVVFIPNYNVS 658
           ++R     PR  + GGKAFATY  AKRIVK + DV A VN+DP++   LKVVFIP+YNVS
Sbjct: 387 KDRINSFVPRVCIFGGKAFATYVQAKRIVKFITDVAATVNHDPEIGDLLKVVFIPDYNVS 446

Query: 659 VAEVLIPGSELSQHISTAGMEASGTSNMKFSLNGCVIIGTLDGANVEIREEVGQENFFLF 718
           VAE LIP SELSQHISTAGMEASGTSNMKF++NGC++IGTLDGANVEIREEVG+ENFFLF
Sbjct: 447 VAEALIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEENFFLF 506

Query: 719 GAKADQVAGLRKDRENGLFKPDPRFEEAKQLIRSGAFGTYDYAPLLDSLEGNSGFGRGDY 778
           GA+A ++AGLRK+R  G F PDPRFEE K+ +RSG FGTY+Y  L+ SLEGN G+GR DY
Sbjct: 507 GAEAHEIAGLRKERAQGKFVPDPRFEEVKRFVRSGVFGTYNYDDLMGSLEGNEGYGRADY 566

Query: 779 FLVGYDFPSYIDAQAQVDEAYKDKKKWIKMSILNTAGSGKFSSDRTIAQYAKEIWGI 835
           FLVG DFPSYI+ Q +VD+AY+D+K W +MSILNTA S KF+SDRTI +YAK+IW I
Sbjct: 567 FLVGKDFPSYIECQEKVDKAYRDQKLWTRMSILNTASSSKFNSDRTIHEYAKDIWDI 623




 Score =  164 bits (415), Expect = 3e-40,   Method: Compositional matrix adjust.
 Identities = 79/131 (60%), Positives = 99/131 (75%), Gaps = 7/131 (5%)

Query: 309 QDIFFRFKERKADRVSGKWSEFPAKVAVQLNDTHPTLAIPELMRLLMDVEGLGWDEAWDI 368
           +DI  RF+ R  D +S  W +FP+KVAVQ+NDTHPTL IPELMR+L+DV+GL W+EAW I
Sbjct: 12  RDIIARFERRAGDSLS--WEDFPSKVAVQMNDTHPTLCIPELMRILIDVKGLSWNEAWSI 69

Query: 369 TNKTIAYTNHTVLPEALEKWSQIVMRKLLPRHMEIIEEIDKRFKEMVIST-----RKEME 423
           T +T+AYTNHTVLPEALEKWS  +M+KLLPRH+EIIE+ID     ++IS         ++
Sbjct: 70  TERTVAYTNHTVLPEALEKWSLDIMQKLLPRHVEIIEKIDGELMNIIISKYGTEDTSLLK 129

Query: 424 GKIDSMRILDN 434
            KI  MRILDN
Sbjct: 130 KKIKEMRILDN 140



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 27,163,342
Number of extensions: 1108478
Number of successful extensions: 2210
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 2208
Number of HSP's successfully gapped: 3
Length of query: 841
Length of database: 17,035,801
Length adjustment: 109
Effective length of query: 732
Effective length of database: 11,344,475
Effective search space: 8304155700
Effective search space used: 8304155700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 160 (66.2 bits)