BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0851600 Os01g0851600|AK101345
         (268 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0851600  3-oxo-5-alpha-steroid 4-dehydrogenase, C-termi...   360   e-100
Os11g0184100  3-oxo-5-alpha-steroid 4-dehydrogenase, C-termi...   291   4e-79
Os07g0162100  3-oxo-5-alpha-steroid 4-dehydrogenase, C-termi...    72   3e-13

>Os01g0851600 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal domain containing
           protein
          Length = 268

 Score =  360 bits (924), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 192/268 (71%), Positives = 192/268 (71%)

Query: 1   MGGGGGDDALYARCLVILYLISPITVFLLRFVSAPYGKLSRPGWGPAVPAALAWFLMESP 60
           MGGGGGDDALYARCLVILYLISPITVFLLRFVSAPYGKLSRPGWGPAVPAALAWFLMESP
Sbjct: 1   MGGGGGDDALYARCLVILYLISPITVFLLRFVSAPYGKLSRPGWGPAVPAALAWFLMESP 60

Query: 61  TMCXXXXXXXXXXXXXXXXXXXXXXXXXXXXHYVNRTLVHXXXXXXXXXXXXXXXILVXX 120
           TMC                            HYVNRTLVH               ILV  
Sbjct: 61  TMCLPPLVLSAAASSSALRAAALLPAALYALHYVNRTLVHPLRLLRLRRAPAPVPILVAA 120

Query: 121 XXXXXXXXXXYVQARSWXXXXXXXXXXXXXXXXXXXXXRCLVGLALFAWGMRTNIAADKA 180
                     YVQARSW                     RCLVGLALFAWGMRTNIAADKA
Sbjct: 121 FAFGFNLLNAYVQARSWALDAAAPHSTATATATPAAVARCLVGLALFAWGMRTNIAADKA 180

Query: 181 LLRLKEAGKGYQIPRGGLFDVVTCPNYFGEAVEWLGYALVAWTPAAWAFFLYTCSNLGPR 240
           LLRLKEAGKGYQIPRGGLFDVVTCPNYFGEAVEWLGYALVAWTPAAWAFFLYTCSNLGPR
Sbjct: 181 LLRLKEAGKGYQIPRGGLFDVVTCPNYFGEAVEWLGYALVAWTPAAWAFFLYTCSNLGPR 240

Query: 241 ARDHRRWYVGKFGDKYPASRKAFVPYIY 268
           ARDHRRWYVGKFGDKYPASRKAFVPYIY
Sbjct: 241 ARDHRRWYVGKFGDKYPASRKAFVPYIY 268



>Os11g0184100 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal domain containing
           protein
          Length = 272

 Score =  291 bits (745), Expect = 4e-79,   Method: Compositional matrix adjust.
 Identities = 158/270 (58%), Positives = 170/270 (62%), Gaps = 8/270 (2%)

Query: 1   MGGGGGDDALYARCLVILYLISPITVFLLRFVSAPYGKLSRPGWGPAVPAALAWFLMESP 60
           MG   GD  LYARCLV LYL+SP+ VF L F+SAPYGKLSRPGWGP VPAALAW LMESP
Sbjct: 9   MGSSDGD-VLYARCLVTLYLLSPLNVFALWFMSAPYGKLSRPGWGPTVPAALAWCLMESP 67

Query: 61  TMCX--XXXXXXXXXXXXXXXXXXXXXXXXXXXHYVNRTLVHXXXXXXXXXXXXXXXILV 118
           T+                               HYV+RTLVH               IL+
Sbjct: 68  TLWLPPLVLSATTTSLSTPTLRVTILPAALYMLHYVHRTLVHPLRLLRLRRAPAPMPILI 127

Query: 119 XXXXXXXXXXXXYVQARSWXXXXXXXXXXXXXXXXXXXXXRCLVGLALFAWGMRTNIAAD 178
                       Y+QARSW                     RCLVGLALF WGM TNIAAD
Sbjct: 128 IMFGFSFNLLNAYIQARSWALDAVPPATATPLTVA-----RCLVGLALFVWGMWTNIAAD 182

Query: 179 KALLRLKEAGKGYQIPRGGLFDVVTCPNYFGEAVEWLGYALVAWTPAAWAFFLYTCSNLG 238
           + LLRLKEAGKGYQIP+ GLFD+V CPNYFGE VEWLGYALVAWTPAAWAFFLYTC NLG
Sbjct: 183 RELLRLKEAGKGYQIPKDGLFDLVACPNYFGETVEWLGYALVAWTPAAWAFFLYTCVNLG 242

Query: 239 PRARDHRRWYVGKFGDKYPASRKAFVPYIY 268
           PRARD R WY+ KFGDKYPASRKAF+PYIY
Sbjct: 243 PRARDQRLWYISKFGDKYPASRKAFIPYIY 272



>Os07g0162100 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal domain containing
           protein
          Length = 277

 Score = 72.4 bits (176), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 46/112 (41%), Positives = 60/112 (53%), Gaps = 8/112 (7%)

Query: 163 GLALFAWGMRTNIAADKALLRLKEAGKG-------YQIPRGGLFDVVTCPNYFGEAVEWL 215
           G+A FA G+  N      L +L+ A  G       Y+IP GGLF +  CP+Y  E V + 
Sbjct: 166 GVAAFAVGIAGNFYHHYLLSQLRNAAGGSGGGERQYRIPTGGLFGLAACPHYLFEIVGFF 225

Query: 216 GYALVAWTPAAWAFFLYTCSNLGPRARDHRRWYVGKFGDKYPASRKAFVPYI 267
           G+A++A T  A A    T + L  R+   RRWY  KF D +P S KA VPYI
Sbjct: 226 GFAMIAQTAHALAVASGTVAYLAGRSCATRRWYESKFED-FPDSIKALVPYI 276



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.143    0.495 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,545,783
Number of extensions: 297988
Number of successful extensions: 884
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 882
Number of HSP's successfully gapped: 4
Length of query: 268
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 169
Effective length of database: 11,866,615
Effective search space: 2005457935
Effective search space used: 2005457935
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 155 (64.3 bits)