BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0816500 Os01g0816500|J080307N12
         (147 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0816500  Similar to Microtubule organization 1 protein ...   231   2e-61
Os01g0816400  Similar to Microtubule bundling polypeptide TM...   170   4e-43

>Os01g0816500 Similar to Microtubule organization 1 protein (MOR1/GEM1)
          Length = 147

 Score =  231 bits (589), Expect = 2e-61,   Method: Compositional matrix adjust.
 Identities = 116/147 (78%), Positives = 116/147 (78%)

Query: 1   VDVWDLLVNFRYAKGICDAIVAKCLTGRPRIVEKAQAALLLWVGLDAAEVFVESMEXXXX 60
           VDVWDLLVNFRYAKGICDAIVAKCLTGRPRIVEKAQAALLLWVGLDAAEVFVESME    
Sbjct: 1   VDVWDLLVNFRYAKGICDAIVAKCLTGRPRIVEKAQAALLLWVGLDAAEVFVESMEKAVK 60

Query: 61  XXXXXXXXXXIDVMFQALSKFGXXXXXXXXXXXXXXXXXDHPDRNVRASSKGLTVELCWW 120
                     IDVMFQALSKFG                 DHPDRNVRASSKGLTVELCWW
Sbjct: 61  NKMAKAVVPAIDVMFQALSKFGPKVVPPKKVLKMLPQLLDHPDRNVRASSKGLTVELCWW 120

Query: 121 IGKEPVKAILFEKIRDMMVGYFIPDHY 147
           IGKEPVKAILFEKIRDMMVGYFIPDHY
Sbjct: 121 IGKEPVKAILFEKIRDMMVGYFIPDHY 147



>Os01g0816400 Similar to Microtubule bundling polypeptide TMBP200
          Length = 1997

 Score =  170 bits (430), Expect = 4e-43,   Method: Composition-based stats.
 Identities = 83/128 (64%), Positives = 91/128 (71%)

Query: 11  RYAKGICDAIVAKCLTGRPRIVEKAQAALLLWVGLDAAEVFVESMEXXXXXXXXXXXXXX 70
           RYAK +CDAIVAKCLTGRP+ VEKAQAA LLWV L+AAEVF+ESME              
Sbjct: 90  RYAKEVCDAIVAKCLTGRPKTVEKAQAAFLLWVELEAAEVFLESMEKAVKNKVAKAVVPA 149

Query: 71  IDVMFQALSKFGXXXXXXXXXXXXXXXXXDHPDRNVRASSKGLTVELCWWIGKEPVKAIL 130
           IDVMFQALS+FG                 DHPD+NVRASSKGLT+ELC WIGKEPVKAIL
Sbjct: 150 IDVMFQALSEFGAKVVPPKKILKMLPELFDHPDQNVRASSKGLTLELCRWIGKEPVKAIL 209

Query: 131 FEKIRDMM 138
           FEK+RD M
Sbjct: 210 FEKMRDTM 217



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.327    0.141    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,378,045
Number of extensions: 84361
Number of successful extensions: 193
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 191
Number of HSP's successfully gapped: 2
Length of query: 147
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 56
Effective length of database: 12,284,327
Effective search space: 687922312
Effective search space used: 687922312
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 151 (62.8 bits)