BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0812600 Os01g0812600|Os01g0812600
         (211 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0812600  NSF attachment protein family protein               324   3e-89
Os01g0809800                                                      113   1e-25

>Os01g0812600 NSF attachment protein family protein
          Length = 211

 Score =  324 bits (830), Expect = 3e-89,   Method: Compositional matrix adjust.
 Identities = 164/211 (77%), Positives = 164/211 (77%)

Query: 1   MGTVXXXXXXXXXXXXXXXXXWVKALDVXXXXXXXXXXXXXXXXXXXXXKGDKLMSQARR 60
           MGTV                 WVKALDV                     KGDKLMSQARR
Sbjct: 1   MGTVSSSSRRRAAGSQPSSSSWVKALDVENDEEAAARADADADAHALEAKGDKLMSQARR 60

Query: 61  ELHGVWSYVSRPFIVAARARFYFHKAAETFVLANSWRKXXXXXXXXXVCCMKIGRSGRLR 120
           ELHGVWSYVSRPFIVAARARFYFHKAAETFVLANSWRK         VCCMKIGRSGRLR
Sbjct: 61  ELHGVWSYVSRPFIVAARARFYFHKAAETFVLANSWRKAAAAHHEHAVCCMKIGRSGRLR 120

Query: 121 AAFALFEAGKCYMKVLEPDDEEMTSRTVSDLEKSLRMFVLENELVMAAEVCVELANLYAM 180
           AAFALFEAGKCYMKVLEPDDEEMTSRTVSDLEKSLRMFVLENELVMAAEVCVELANLYAM
Sbjct: 121 AAFALFEAGKCYMKVLEPDDEEMTSRTVSDLEKSLRMFVLENELVMAAEVCVELANLYAM 180

Query: 181 LKQWEKVREYRQKAAEFHAKTSDALFDTSTV 211
           LKQWEKVREYRQKAAEFHAKTSDALFDTSTV
Sbjct: 181 LKQWEKVREYRQKAAEFHAKTSDALFDTSTV 211



>Os01g0809800 
          Length = 175

 Score =  113 bits (282), Expect = 1e-25,   Method: Compositional matrix adjust.
 Identities = 59/146 (40%), Positives = 86/146 (58%)

Query: 50  KGDKLMSQARRELHGVWSYVSRPFIVAARARFYFHKAAETFVLANSWRKXXXXXXXXXVC 109
           KGD+LM+QARREL G WS +       + A  +F +AA+ + LA +WRK           
Sbjct: 11  KGDRLMAQARRELTGFWSCLLPASATYSYAAGFFEEAAKAYRLAKNWRKAALAHHEYGTY 70

Query: 110 CMKIGRSGRLRAAFALFEAGKCYMKVLEPDDEEMTSRTVSDLEKSLRMFVLENELVMAAE 169
           C+K+GR  RL AA  L+E+ +C+M+  +PDDE+      SDL++S+RM VLEN+  +AA 
Sbjct: 71  CIKMGRDCRLAAAVVLWESAECHMRDFDPDDEQTARAIESDLKRSVRMLVLENQPQLAAS 130

Query: 170 VCVELANLYAMLKQWEKVREYRQKAA 195
            C ELA +Y   ++     E R  A+
Sbjct: 131 ACEELARMYVARRRGSGTSEPRGTAS 156



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.132    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,558,690
Number of extensions: 129449
Number of successful extensions: 503
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 506
Number of HSP's successfully gapped: 2
Length of query: 211
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 115
Effective length of database: 12,023,257
Effective search space: 1382674555
Effective search space used: 1382674555
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 154 (63.9 bits)