BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0167400 Os01g0167400|AB051107
         (270 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0167400  Similar to Protein synthesis inhibitor II (EC ...   414   e-116
Os01g0160800  Similar to Protein synthesis inhibitor II (EC ...   210   7e-55

>Os01g0167400 Similar to Protein synthesis inhibitor II (EC 3.2.2.22)
           (Ribosome-inactivating protein II) (rRNA N-glycosidase)
          Length = 270

 Score =  414 bits (1063), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 215/270 (79%), Positives = 215/270 (79%)

Query: 1   MALNPLFTVTFDVSSGDNYGDFIAGIRSRVANPRHFSXXXXXXXXXXXXXXXXXXXXXXL 60
           MALNPLFTVTFDVSSGDNYGDFIAGIRSRVANPRHFS                      L
Sbjct: 1   MALNPLFTVTFDVSSGDNYGDFIAGIRSRVANPRHFSRNRPVLPPVEPPPPPRRWFHVVL 60

Query: 61  RASPTAALTLATRADNLYLEGFRSSDGRWWEXXXXXXXXXXXXXXXXYVGFGGSYRDLLG 120
           RASPTAALTLATRADNLYLEGFRSSDGRWWE                YVGFGGSYRDLLG
Sbjct: 61  RASPTAALTLATRADNLYLEGFRSSDGRWWELTPGILGAAPGGAAATYVGFGGSYRDLLG 120

Query: 121 DTDRLTVVTLGPQQMAQAVNALAARRPADLANGXXXXXXXXXXXXXXXXXHEATRFQTVS 180
           DTDRLTVVTLGPQQMAQAVNALAARRPADLANG                 HEATRFQTVS
Sbjct: 121 DTDRLTVVTLGPQQMAQAVNALAARRPADLANGAAQRRAMDAVAALLLMVHEATRFQTVS 180

Query: 181 RLVAGLMHPKAASKSGAITTAMRKQVNGWQVLSAAMLGTDARPPARFAPLRDMGVDTVEE 240
           RLVAGLMHPKAASKSGAITTAMRKQVNGWQVLSAAMLGTDARPPARFAPLRDMGVDTVEE
Sbjct: 181 RLVAGLMHPKAASKSGAITTAMRKQVNGWQVLSAAMLGTDARPPARFAPLRDMGVDTVEE 240

Query: 241 AAATVGILLFVEVPGGMTAARALQLFHHGN 270
           AAATVGILLFVEVPGGMTAARALQLFHHGN
Sbjct: 241 AAATVGILLFVEVPGGMTAARALQLFHHGN 270



>Os01g0160800 Similar to Protein synthesis inhibitor II (EC 3.2.2.22)
           (Ribosome-inactivating protein II) (rRNA N-glycosidase)
          Length = 282

 Score =  210 bits (535), Expect = 7e-55,   Method: Compositional matrix adjust.
 Identities = 142/289 (49%), Positives = 161/289 (55%), Gaps = 31/289 (10%)

Query: 1   MALNPLFTVTFDVSSG--DNYGDFIAGIRSRVANPRHFSXXXXXXXXXXXXXXXXXXXXX 58
           MALNPLFTVTF+VS    DNYGDFIAGIR RVANPRHFS                     
Sbjct: 1   MALNPLFTVTFNVSGSGSDNYGDFIAGIRKRVANPRHFSHNRPVLPPVEPDVPPRRWFHV 60

Query: 59  XLRASPTAALTLATRADNLYLEGFRSSDGR--WWEXXXXXXXXXXXXXXXXYVGFGGSYR 116
            LR + T+ LTLATRADNLYLEGFR SDG   WWE                Y+GFGGSYR
Sbjct: 61  VLR-TQTSELTLATRADNLYLEGFRGSDGTSAWWELTRGLIAGAT------YLGFGGSYR 113

Query: 117 DLLGDTDRLTVVTLGPQQMAQAVNALAARRPADLANGXXXXXXXXXXXXXXXXXHEATRF 176
           +LLG TD +  VTLGPQQM QAV+ LA    A    G                 +EA RF
Sbjct: 114 ELLGHTDNMVGVTLGPQQMTQAVDTLAGL--AASGGGAARQRAGEALATLLLMVNEAVRF 171

Query: 177 QTVSRLVAGLMHPKAASKSGAITTAMRKQVNGWQVLSAAMLGTDA-RPPARFAPLRDMGV 235
            TV+ LV G M+P+A  KSG IT  M++QVNGW+VLS A+L  DA +     +  +   V
Sbjct: 172 LTVAELVGGFMNPRAVRKSGTITADMKEQVNGWKVLSRALLTMDALQLEDSNSASKHNKV 231

Query: 236 DTVE----------------EAAATVGILLFVE-VPGGMTAARALQLFH 267
           DT +                EAA  VGILLFVE VP GMT A ALQLFH
Sbjct: 232 DTKKMEQEKKAWEAAEKLAVEAAKAVGILLFVEKVPAGMTKATALQLFH 280



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.133    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,870,493
Number of extensions: 208129
Number of successful extensions: 524
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 515
Number of HSP's successfully gapped: 2
Length of query: 270
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 171
Effective length of database: 11,866,615
Effective search space: 2029191165
Effective search space used: 2029191165
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)