BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0166300 Os01g0166300|J065188L13
(105 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0166300 Hypothetical protein 177 2e-45
Os05g0165400 Conserved hypothetical protein 79 1e-15
>Os01g0166300 Hypothetical protein
Length = 105
Score = 177 bits (448), Expect = 2e-45, Method: Compositional matrix adjust.
Identities = 88/105 (83%), Positives = 88/105 (83%)
Query: 1 MQSFSGIQALQRHFPCFLDXXXXXXXXXXXXXXXXXKATFYSGRVHRQFVPVGKLLKPVL 60
MQSFSGIQALQRHFPCFLD KATFYSGRVHRQFVPVGKLLKPVL
Sbjct: 1 MQSFSGIQALQRHFPCFLDFIYAAARRFIFFYLLFIKATFYSGRVHRQFVPVGKLLKPVL 60
Query: 61 NEHVTPITCYWSLVLLLHSEDKLVRVFKVNFSCFQLVSSALRGIR 105
NEHVTPITCYWSLVLLLHSEDKLVRVFKVNFSCFQLVSSALRGIR
Sbjct: 61 NEHVTPITCYWSLVLLLHSEDKLVRVFKVNFSCFQLVSSALRGIR 105
>Os05g0165400 Conserved hypothetical protein
Length = 201
Score = 78.6 bits (192), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 34/49 (69%), Positives = 43/49 (87%)
Query: 40 FYSGRVHRQFVPVGKLLKPVLNEHVTPITCYWSLVLLLHSEDKLVRVFK 88
F+SGRVHR+FVP+GK+LKP+LNE VTP+TCYWSL LLL E++L VF+
Sbjct: 115 FWSGRVHRRFVPIGKILKPLLNECVTPVTCYWSLALLLRDEEELRLVFQ 163
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.331 0.142 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,603,726
Number of extensions: 73786
Number of successful extensions: 141
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 140
Number of HSP's successfully gapped: 2
Length of query: 105
Length of database: 17,035,801
Length adjustment: 73
Effective length of query: 32
Effective length of database: 13,224,179
Effective search space: 423173728
Effective search space used: 423173728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 149 (62.0 bits)