BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0160800 Os01g0160800|AB051108
         (282 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0160800  Similar to Protein synthesis inhibitor II (EC ...   437   e-123
Os01g0167400  Similar to Protein synthesis inhibitor II (EC ...   249   2e-66

>Os01g0160800 Similar to Protein synthesis inhibitor II (EC 3.2.2.22)
           (Ribosome-inactivating protein II) (rRNA N-glycosidase)
          Length = 282

 Score =  437 bits (1125), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 223/282 (79%), Positives = 223/282 (79%)

Query: 1   MALNPLFTVTFNVSGSGSDNYGDFIAGIRKRVANPRHFSHNXXXXXXXXXXXXXXXWFHV 60
           MALNPLFTVTFNVSGSGSDNYGDFIAGIRKRVANPRHFSHN               WFHV
Sbjct: 1   MALNPLFTVTFNVSGSGSDNYGDFIAGIRKRVANPRHFSHNRPVLPPVEPDVPPRRWFHV 60

Query: 61  VLRTQTSELTLATRADNLYLEGFRGSDGTSAWWELTRGLIAGATYLGFGGSYRELLGHTD 120
           VLRTQTSELTLATRADNLYLEGFRGSDGTSAWWELTRGLIAGATYLGFGGSYRELLGHTD
Sbjct: 61  VLRTQTSELTLATRADNLYLEGFRGSDGTSAWWELTRGLIAGATYLGFGGSYRELLGHTD 120

Query: 121 NMVGVTLGPQQMTQAVDTXXXXXXXXXXXXXXXXXXXXXTLLLMVNEAVRFLTVAELVGG 180
           NMVGVTLGPQQMTQAVDT                     TLLLMVNEAVRFLTVAELVGG
Sbjct: 121 NMVGVTLGPQQMTQAVDTLAGLAASGGGAARQRAGEALATLLLMVNEAVRFLTVAELVGG 180

Query: 181 FMNPRAVRKSGTITADMKEQVNGWKVLSRALLTMDALQLEDSNSASKHNKVDTXXXXXXX 240
           FMNPRAVRKSGTITADMKEQVNGWKVLSRALLTMDALQLEDSNSASKHNKVDT       
Sbjct: 181 FMNPRAVRKSGTITADMKEQVNGWKVLSRALLTMDALQLEDSNSASKHNKVDTKKMEQEK 240

Query: 241 XXXXXXXXXXXXXXXXVGILLFVEKVPAGMTKATALQLFHGN 282
                           VGILLFVEKVPAGMTKATALQLFHGN
Sbjct: 241 KAWEAAEKLAVEAAKAVGILLFVEKVPAGMTKATALQLFHGN 282



>Os01g0167400 Similar to Protein synthesis inhibitor II (EC 3.2.2.22)
           (Ribosome-inactivating protein II) (rRNA N-glycosidase)
          Length = 270

 Score =  249 bits (635), Expect = 2e-66,   Method: Compositional matrix adjust.
 Identities = 151/289 (52%), Positives = 171/289 (59%), Gaps = 31/289 (10%)

Query: 1   MALNPLFTVTFNVSGSGSDNYGDFIAGIRKRVANPRHFSHNXXXXXXXXXXXXXXXWFHV 60
           MALNPLFTVTF+VS    DNYGDFIAGIR RVANPRHFS N               WFHV
Sbjct: 1   MALNPLFTVTFDVSSG--DNYGDFIAGIRSRVANPRHFSRNRPVLPPVEPPPPPRRWFHV 58

Query: 61  VLR-TQTSELTLATRADNLYLEGFRGSDGTSAWWELTRGLIAGA------TYLGFGGSYR 113
           VLR + T+ LTLATRADNLYLEGFR SDG   WWELT G++  A      TY+GFGGSYR
Sbjct: 59  VLRASPTAALTLATRADNLYLEGFRSSDGR--WWELTPGILGAAPGGAAATYVGFGGSYR 116

Query: 114 ELLGHTDNMVGVTLGPQQMTQAVDTXXXXXXXXXX--XXXXXXXXXXXTLLLMVNEAVRF 171
           +LLG TD +  VTLGPQQM QAV+                         LLLMV+EA RF
Sbjct: 117 DLLGDTDRLTVVTLGPQQMAQAVNALAARRPADLANGAAQRRAMDAVAALLLMVHEATRF 176

Query: 172 LTVAELVGGFMNPRAVRKSGTITADMKEQVNGWKVLSRALLTMDALQLEDSNSASKHNKV 231
            TV+ LV G M+P+A  KSG IT  M++QVNGW+VLS A+L  DA +     +  +   V
Sbjct: 177 QTVSRLVAGLMHPKAASKSGAITTAMRKQVNGWQVLSAAMLGTDA-RPPARFAPLRDMGV 235

Query: 232 DTXXXXXXXXXXXXXXXXXXXXXXXVGILLFVEKVPAGMTKATALQLFH 280
           DT                       VGILLFVE VP GMT A ALQLFH
Sbjct: 236 DT----------------VEEAAATVGILLFVE-VPGGMTAARALQLFH 267



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,701,647
Number of extensions: 201558
Number of successful extensions: 437
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 431
Number of HSP's successfully gapped: 2
Length of query: 282
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 182
Effective length of database: 11,814,401
Effective search space: 2150220982
Effective search space used: 2150220982
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 155 (64.3 bits)