BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0565600 Os09g0565600|J075047H16
         (240 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0565600  Similar to Delta(14)-sterol reductase (EC 1.3....   489   e-139
Os02g0465400  Similar to 7-dehydrocholesterol reductase (EC ...   120   1e-27

>Os09g0565600 Similar to Delta(14)-sterol reductase (EC 1.3.1.70) (C-14 sterol
           reductase) (Sterol C14-reductase) (FACKEL protein)
          Length = 240

 Score =  489 bits (1258), Expect = e-139,   Method: Compositional matrix adjust.
 Identities = 240/240 (100%), Positives = 240/240 (100%)

Query: 1   WLGVQLNPHFMGVDLKFFFVRAGMMAWLFINLSLFAKSYLAGSANLSVILYQFFCAWYIV 60
           WLGVQLNPHFMGVDLKFFFVRAGMMAWLFINLSLFAKSYLAGSANLSVILYQFFCAWYIV
Sbjct: 1   WLGVQLNPHFMGVDLKFFFVRAGMMAWLFINLSLFAKSYLAGSANLSVILYQFFCAWYIV 60

Query: 61  DYFVHEEFMTSTWDIIAERLGFMLVFGDLVFIPFTFTIQGWWLLRNKVELSLLAAVVNCF 120
           DYFVHEEFMTSTWDIIAERLGFMLVFGDLVFIPFTFTIQGWWLLRNKVELSLLAAVVNCF
Sbjct: 61  DYFVHEEFMTSTWDIIAERLGFMLVFGDLVFIPFTFTIQGWWLLRNKVELSLLAAVVNCF 120

Query: 121 IFVIGYLVFRGANKQKHIFKKNPKALIWGKPPKLVGGKLLVSGYWGIARHCNYLGDILLA 180
           IFVIGYLVFRGANKQKHIFKKNPKALIWGKPPKLVGGKLLVSGYWGIARHCNYLGDILLA
Sbjct: 121 IFVIGYLVFRGANKQKHIFKKNPKALIWGKPPKLVGGKLLVSGYWGIARHCNYLGDILLA 180

Query: 181 LSFSLPCGTSSVIPYFYPTYLFILLIWRERRDEARCSEKYKEIWVEYCKLVPWRIFPYVY 240
           LSFSLPCGTSSVIPYFYPTYLFILLIWRERRDEARCSEKYKEIWVEYCKLVPWRIFPYVY
Sbjct: 181 LSFSLPCGTSSVIPYFYPTYLFILLIWRERRDEARCSEKYKEIWVEYCKLVPWRIFPYVY 240



>Os02g0465400 Similar to 7-dehydrocholesterol reductase (EC 1.3.1.21) (7-DHC
           reductase) (Sterol delta-7-reductase) (Dwarf5 protein)
          Length = 450

 Score =  120 bits (300), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 82/257 (31%), Positives = 130/257 (50%), Gaps = 22/257 (8%)

Query: 3   GVQLNP----HFMGVDLKFFF-VRAGMMAWLFINLSLFAKSY-LAGSANLSVILYQFFCA 56
           G++L P    HF   D+K F   R GMM+W  + ++   K Y + G    S+++      
Sbjct: 197 GMELYPRIGKHF---DIKVFTNCRFGMMSWAVLAVTYCIKQYEMNGRVADSMLVNTALML 253

Query: 57  WYIVDYFVHEEFMTSTWDIIAERLGFMLVFGDLVFIPFTFTIQGWWLLRNKVELSLLAAV 116
            Y+  +F  E     T DI  +R GF + +G LV++P  +T  G +L+ + V L    A+
Sbjct: 254 IYVTKFFWWESGYWCTMDIAHDRAGFYICWGCLVWVPSIYTSPGMYLVNHPVNLGPQLAL 313

Query: 117 VNCFIFVIGYLVFRGANKQKHIFKK-NPKALIWGKPPKLV------------GGKLLVSG 163
                 ++   +    ++Q+  F++ N K  IWGK P  +               LL SG
Sbjct: 314 SILLAGILCIYINYDCDRQRQEFRRTNGKCSIWGKAPSKIVASYQTTNGETKSSLLLTSG 373

Query: 164 YWGIARHCNYLGDILLALSFSLPCGTSSVIPYFYPTYLFILLIWRERRDEARCSEKYKEI 223
           +WG++RH +Y+ +IL A  +++P      +PYFY  +L ILL  R +RD+ RCS KY + 
Sbjct: 374 WWGLSRHFHYVPEILSAFFWTVPALFDHFLPYFYVIFLTILLFDRAKRDDDRCSSKYGKY 433

Query: 224 WVEYCKLVPWRIFPYVY 240
           W  YC  VP R+ P +Y
Sbjct: 434 WKMYCNKVPCRVIPGIY 450



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.332    0.147    0.508 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,315,378
Number of extensions: 329390
Number of successful extensions: 683
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 681
Number of HSP's successfully gapped: 2
Length of query: 240
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 142
Effective length of database: 11,918,829
Effective search space: 1692473718
Effective search space used: 1692473718
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 154 (63.9 bits)