BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0580000 Os01g0580000|AK121186
         (324 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0580000  Esterase/lipase/thioesterase domain containing...   523   e-149
Os01g0579900  Esterase/lipase/thioesterase domain containing...   360   e-100
Os05g0539500  Esterase/lipase/thioesterase domain containing...   278   4e-75

>Os01g0580000 Esterase/lipase/thioesterase domain containing protein
          Length = 324

 Score =  523 bits (1346), Expect = e-149,   Method: Compositional matrix adjust.
 Identities = 271/287 (94%), Positives = 271/287 (94%)

Query: 38  GALLCNRTGSGHASPRGRRPLPPPAMSHPLSSEPHALEQRAVVVTNKHGEKLVGVLHHTG 97
           GALLCNRTGSGHASPRGRRPLPPPAMSHPLSSEPHALEQRAVVVTNKHGEKLVGVLHHTG
Sbjct: 38  GALLCNRTGSGHASPRGRRPLPPPAMSHPLSSEPHALEQRAVVVTNKHGEKLVGVLHHTG 97

Query: 98  SSKIVVLCHGFISTKNDSLILDLMAALTKKGISVFRFDXXXXXXXXXXXXXXXXRKEADD 157
           SSKIVVLCHGFISTKNDSLILDLMAALTKKGISVFRFD                RKEADD
Sbjct: 98  SSKIVVLCHGFISTKNDSLILDLMAALTKKGISVFRFDFSGNGESEGEFEYGNYRKEADD 157

Query: 158 LHSVVSYLCKEKYDVTAIVGHSKGGDVVTLYASIYDDVRLVINVSGRFDLEKGIEERIGE 217
           LHSVVSYLCKEKYDVTAIVGHSKGGDVVTLYASIYDDVRLVINVSGRFDLEKGIEERIGE
Sbjct: 158 LHSVVSYLCKEKYDVTAIVGHSKGGDVVTLYASIYDDVRLVINVSGRFDLEKGIEERIGE 217

Query: 218 GSIDRINKEGYLDVKDKSGNVQYRVTKESLMERLNTDIRAVSMSITKECRFFTVHGSADE 277
           GSIDRINKEGYLDVKDKSGNVQYRVTKESLMERLNTDIRAVSMSITKECRFFTVHGSADE
Sbjct: 218 GSIDRINKEGYLDVKDKSGNVQYRVTKESLMERLNTDIRAVSMSITKECRFFTVHGSADE 277

Query: 278 TIPVEDAYKFAKHIPNHKLQVIEGANHNYTAHREELADAVVDFITSN 324
           TIPVEDAYKFAKHIPNHKLQVIEGANHNYTAHREELADAVVDFITSN
Sbjct: 278 TIPVEDAYKFAKHIPNHKLQVIEGANHNYTAHREELADAVVDFITSN 324



>Os01g0579900 Esterase/lipase/thioesterase domain containing protein
          Length = 275

 Score =  360 bits (925), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 179/252 (71%), Positives = 208/252 (82%), Gaps = 1/252 (0%)

Query: 73  ALEQRAVVVTNKHGEKLVGVLHHTGSSKIVVLCHGFISTKNDSLILDLMAALTKKGISVF 132
           A E+R VVVTNKHGEKLVG+L H GS+KIVV+CHGF ++KNDS+I+DL  ALTKKG+ +F
Sbjct: 17  AQEER-VVVTNKHGEKLVGLLQHMGSNKIVVICHGFTASKNDSIIVDLANALTKKGVGIF 75

Query: 133 RFDXXXXXXXXXXXXXXXXRKEADDLHSVVSYLCKEKYDVTAIVGHSKGGDVVTLYASIY 192
           RFD                RKEADDLHSV+S+L +EKYDV AIVGHSKGGDVV LYASIY
Sbjct: 76  RFDFSGNGESEGEFQYGNYRKEADDLHSVISHLNQEKYDVKAIVGHSKGGDVVVLYASIY 135

Query: 193 DDVRLVINVSGRFDLEKGIEERIGEGSIDRINKEGYLDVKDKSGNVQYRVTKESLMERLN 252
           DDVR V+N+SGRF LEKGIEER+G+  ++ I+KEGY+DVK  SG V Y+VTKESLMERL 
Sbjct: 136 DDVRTVVNLSGRFHLEKGIEERLGKEFMNIIDKEGYIDVKTNSGKVLYKVTKESLMERLT 195

Query: 253 TDIRAVSMSITKECRFFTVHGSADETIPVEDAYKFAKHIPNHKLQVIEGANHNYTAHREE 312
           TD+    +SI+KECRFFTVHGSADE IPVEDAY+FAKHIPNHKL VIEGANH YTAHR+E
Sbjct: 196 TDMHKACLSISKECRFFTVHGSADEIIPVEDAYEFAKHIPNHKLHVIEGANHCYTAHRKE 255

Query: 313 LADAVVDFITSN 324
           L+DAVVDFITS+
Sbjct: 256 LSDAVVDFITSS 267



>Os05g0539500 Esterase/lipase/thioesterase domain containing protein
          Length = 282

 Score =  278 bits (711), Expect = 4e-75,   Method: Compositional matrix adjust.
 Identities = 131/263 (49%), Positives = 184/263 (69%)

Query: 61  PAMSHPLSSEPHALEQRAVVVTNKHGEKLVGVLHHTGSSKIVVLCHGFISTKNDSLILDL 120
           P MS   S E   + ++ V ++NKHGE LVG+LH   S  +V+LCHGF +TK+DS+++DL
Sbjct: 8   PFMSPEKSPESSVVREQRVTISNKHGENLVGLLHQACSKNLVILCHGFRATKDDSILVDL 67

Query: 121 MAALTKKGISVFRFDXXXXXXXXXXXXXXXXRKEADDLHSVVSYLCKEKYDVTAIVGHSK 180
             ALT++G+S FRFD                R+EADDLHSVVSY  +++Y++  +VGHSK
Sbjct: 68  AYALTREGVSAFRFDFAGNGESEGQFQYGNYRREADDLHSVVSYFTEQEYNIIGLVGHSK 127

Query: 181 GGDVVTLYASIYDDVRLVINVSGRFDLEKGIEERIGEGSIDRINKEGYLDVKDKSGNVQY 240
           GG+ V LYAS+  D+ +++N+SGRF LE+GI+ R+G+  + RI K+GY+DV+++ G  +Y
Sbjct: 128 GGNAVLLYASMNHDIPVIVNISGRFALERGIDGRLGKNFMQRIKKDGYIDVRNRKGEFEY 187

Query: 241 RVTKESLMERLNTDIRAVSMSITKECRFFTVHGSADETIPVEDAYKFAKHIPNHKLQVIE 300
           +VT+ESL +RL+TD    S SI+K CR  T+HGS DE +PVEDA  FA +IPNH+L +I 
Sbjct: 188 QVTEESLKDRLSTDTLLSSRSISKCCRVLTIHGSKDEIVPVEDALMFAANIPNHELHIIA 247

Query: 301 GANHNYTAHREELADAVVDFITS 323
            ANH YT H +EL   V+DFI S
Sbjct: 248 EANHRYTGHEKELKAFVLDFIKS 270



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,394,600
Number of extensions: 373927
Number of successful extensions: 1624
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1622
Number of HSP's successfully gapped: 3
Length of query: 324
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 223
Effective length of database: 11,762,187
Effective search space: 2622967701
Effective search space used: 2622967701
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)