BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0621700 Os12g0621700|AK073528
         (620 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G54750.3  | chr3:20264833-20268416 REVERSE LENGTH=591          366   e-101

>AT3G54750.3 | chr3:20264833-20268416 REVERSE LENGTH=591
          Length = 590

 Score =  366 bits (940), Expect = e-101,   Method: Compositional matrix adjust.
 Identities = 233/579 (40%), Positives = 318/579 (54%), Gaps = 58/579 (10%)

Query: 26  KRKTPSELRGEQLKRRA---SDKPANDQSLHSAAFDRASNGFRNPEQSKISKYISTRVTE 82
           KRKTPSELRGEQLKR A     K A D      +     NGF+  E SK  KYI  R+ E
Sbjct: 7   KRKTPSELRGEQLKRTAFVDQAKEAFDALRPCKSSAERENGFKKSELSKNPKYIEMRMDE 66

Query: 83  VFPVKKSRNL---GKENFKNNEKAPKSADAVTTSDFASPSLPCG--YGDSAKLDSAVPSH 137
           ++PVKK+R     GKEN K N  A +S+  +  S  ++ +        +     + VP  
Sbjct: 67  LYPVKKARPWMLSGKENSKEN-GAKQSSSLINVSLLSNLAATKRQLIREENNASTEVPDD 125

Query: 138 TEAAKPSFRKVEKCSENALRSVSELHIGDEQQSGTNKFDMEKVLKGFGARDAFVASKLTD 197
           T+A   + +  E+CS++  RSV++L    E+ S     DM K LKG    +      L  
Sbjct: 126 TKAE--ARQTNERCSQSIFRSVTQLSTRGEELSCLPDIDMNKALKGLATCEPL----LVH 179

Query: 198 PNIQVGVVPSKSLDLC-PSEIAVPGKRAPLDLTLKTTLQFVSSSSVKWCHKISTSFGRSS 256
           P    G   + SL     SE  V G++ PLDL+LKT  + VSSS + W H+        S
Sbjct: 180 PGDITGEDDTASLSGSFMSEFQVSGQKIPLDLSLKTYARLVSSSPLSWLHR--------S 231

Query: 257 IVGP----IAQSYHHGCQ-----NSGCSRPERNKEFLFSKALQSWVYPQSLLPASIISAM 307
           I+G     + Q     C      NS  S      + L S +L SWVYPQS LP  +ISAM
Sbjct: 232 IMGSTYNGMPQLKSLSCNVVNQDNSSGSGASVVSQVLNSMSLHSWVYPQSTLPPFVISAM 291

Query: 308 VSSTV-RGESDFLLKRHQDWEDSFQNLYYMLRKNLLNIFYVYTPQFVALFIGGNCLD--K 364
           V+S   RGE DFL KR   WED+F++LY+M RKNL  +FYV T QFVA+F G +C     
Sbjct: 292 VASVSDRGEVDFLQKRKLAWEDAFRSLYFMFRKNLCKVFYVCTSQFVAMFTG-SCESGGA 350

Query: 365 KQTCNAYLSQSTRGIRSLLRRHGVCFSMPLCNTEVEQVTEDDLIELSEIQKRNLGQALHI 424
           K++CN Y++QSTR +R++L+   +C+SMPLC T+++Q T +DL ELSE++  N+GQ    
Sbjct: 351 KRSCNGYITQSTRRLRAMLKNLDICYSMPLCKTKMDQTTVEDLAELSELENHNIGQIRRS 410

Query: 425 DAMSEVDNTTQSLLSFVGNKSVHGLYDFLLNYKSFLNSLSATDIPVLYSPVPFQNGCLHI 484
            ++S +DNT +S L+FVGN+SVHGLYD LLNY+     L   D+PVL SPVPFQN  L  
Sbjct: 411 RSVSNIDNTPESFLAFVGNESVHGLYDLLLNYRYSFEFLPTADVPVLSSPVPFQNAALSS 470

Query: 485 PEVKCKEMRKADIGLVSGGFDAGEPGSTFASLTGNICYSMEIKDPVLPPWVVSGICAAMS 544
           PE+KC EM K +                        CY +EIK   +PPW++S ICA + 
Sbjct: 471 PEIKCSEMVKTE---------------------HTSCYMVEIKGEYIPPWIISNICANVG 509

Query: 545 SDANSFDLMMATEPSSMGLNAALSCVSSNSQSKAHSSEG 583
           ++  +F+    TEP+S+ LN  L  V   +  ++   EG
Sbjct: 510 ANGQNFEASFVTEPTSVNLNIGLPQVPEKTDPESRVIEG 548



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.130    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 13,049,073
Number of extensions: 551826
Number of successful extensions: 1384
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1377
Number of HSP's successfully gapped: 1
Length of query: 620
Length of database: 11,106,569
Length adjustment: 105
Effective length of query: 515
Effective length of database: 8,227,889
Effective search space: 4237362835
Effective search space used: 4237362835
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 115 (48.9 bits)