BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0615400 Os12g0615400|AK103998
         (348 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G63410.1  | chr3:23415816-23417002 REVERSE LENGTH=339          459   e-129

>AT3G63410.1 | chr3:23415816-23417002 REVERSE LENGTH=339
          Length = 338

 Score =  459 bits (1180), Expect = e-129,   Method: Compositional matrix adjust.
 Identities = 232/333 (69%), Positives = 260/333 (78%), Gaps = 7/333 (2%)

Query: 21  GRIRPPRGLGFSTTTTKSR----PLVLTRRGGGGGNISVARLRCXXXXXXXXXRPMSQPR 76
           G I  P+GLG   +   +R    P +L+        +SVA  RC            +QPR
Sbjct: 8   GAITFPKGLGSPGSNLHARSIPRPTLLSVTRTSTPRLSVA-TRCSSSSVSSSRP-SAQPR 65

Query: 77  FIQHKKEAFWFYRFLSIVYDHVINPGHWTEDMRDDALEPADLYSRKLRVVDVXXXXXXXX 136
           FIQHKKEA+WFYRFLSIVYDHVINPGHWTEDMRDDALEPADL    +RVVDV        
Sbjct: 66  FIQHKKEAYWFYRFLSIVYDHVINPGHWTEDMRDDALEPADLSHPDMRVVDVGGGTGFTT 125

Query: 137 XXIVKRVDPENVTLLDQSPHQLEKAREKEALKGVTIMEGDAEDLPFPTDTFDRYVSAGSI 196
             IVK V  +NVT+LDQSPHQL KA++KE LK   I+EGDAEDLPFPTD  DRYVSAGSI
Sbjct: 126 LGIVKTVKAKNVTILDQSPHQLAKAKQKEPLKECKIVEGDAEDLPFPTDYADRYVSAGSI 185

Query: 197 EYWPDPQRGIKEAYRVLRLGGVACMIGPVHPTFWLSRFFADMWMLFPKEEEYIEWFKKAG 256
           EYWPDPQRGI+EAYRVL++GG AC+IGPV+PTFWLSRFF+D+WMLFPKEEEYIEWFK AG
Sbjct: 186 EYWPDPQRGIREAYRVLKIGGKACLIGPVYPTFWLSRFFSDVWMLFPKEEEYIEWFKNAG 245

Query: 257 FKDVKLKRIGPKWYRGVRRHGLIMGCSVTGVKREHGDSPLQLGPKVEDVSKPV-NPITFL 315
           FKDV+LKRIGPKWYRGVRRHGLIMGCSVTGVK   GDSPLQLGPK EDV KPV NP +FL
Sbjct: 246 FKDVQLKRIGPKWYRGVRRHGLIMGCSVTGVKPASGDSPLQLGPKEEDVEKPVNNPFSFL 305

Query: 316 FRFLMGTICAAYYVLVPIYMWIKDQIVPKGMPI 348
            RFL+GT+ AA++VL+PIYMWIKDQIVPK  PI
Sbjct: 306 GRFLLGTLAAAWFVLIPIYMWIKDQIVPKDQPI 338



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.141    0.452 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,858,413
Number of extensions: 338952
Number of successful extensions: 689
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 691
Number of HSP's successfully gapped: 1
Length of query: 348
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 249
Effective length of database: 8,392,385
Effective search space: 2089703865
Effective search space used: 2089703865
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)