BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0614000 Os12g0614000|AK068768
         (231 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G19480.1  | chr5:6571828-6572929 FORWARD LENGTH=208            152   2e-37
AT5G12230.1  | chr5:3953915-3955285 REVERSE LENGTH=222            147   4e-36

>AT5G19480.1 | chr5:6571828-6572929 FORWARD LENGTH=208
          Length = 207

 Score =  152 bits (383), Expect = 2e-37,   Method: Compositional matrix adjust.
 Identities = 75/115 (65%), Positives = 92/115 (80%), Gaps = 3/115 (2%)

Query: 12  MDSDDKKFGKGPRELTGAVDLISHYKLLAHHDFFCKKPLPLAISDTHYLDNVVGDTEIRK 71
           M+S+  KFG GPREL GA+DLI+ YKLL HH+FFCK+ LP ++SD HYL N+VGDTEIRK
Sbjct: 1   MESESVKFG-GPRELGGALDLITQYKLLPHHEFFCKRSLPESLSDAHYLHNLVGDTEIRK 59

Query: 72  GEGMELDQLVQNAYL--RDKPAYIQPFDMETLGQAFQLRETAPVDLPSAEKGIPT 124
           GEGM+LDQL+ NA L  RD  A IQPF ++ L +AF+L +TAPV+LP AEKG PT
Sbjct: 60  GEGMQLDQLIPNASLSSRDTNARIQPFVLDELKEAFELNDTAPVELPPAEKGAPT 114



>AT5G12230.1 | chr5:3953915-3955285 REVERSE LENGTH=222
          Length = 221

 Score =  147 bits (372), Expect = 4e-36,   Method: Compositional matrix adjust.
 Identities = 71/118 (60%), Positives = 92/118 (77%), Gaps = 2/118 (1%)

Query: 12  MDSDDKKFGKGPRELTGAVDLISHYKLLAHHDFFCKKPLPLAISDTHYLDNVVGDTEIRK 71
           M+ +  KFG GPREL GA DLIS +KL+ HH+FFCKK LP+++SD+HYL NVVGDTEIRK
Sbjct: 1   MEPERLKFG-GPRELCGAADLISQFKLVQHHEFFCKKSLPVSLSDSHYLHNVVGDTEIRK 59

Query: 72  GEGMELDQLVQN-AYLRDKPAYIQPFDMETLGQAFQLRETAPVDLPSAEKGIPTISGK 128
           GEGM+LDQL+++ +  R+    IQPFD++ L ++FQL +  PV+LP AEKG PTI  K
Sbjct: 60  GEGMQLDQLIESISQSRETNIRIQPFDIDELQESFQLNDMTPVELPPAEKGAPTIPSK 117



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.139    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,375,908
Number of extensions: 123161
Number of successful extensions: 195
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 193
Number of HSP's successfully gapped: 2
Length of query: 231
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 136
Effective length of database: 8,502,049
Effective search space: 1156278664
Effective search space used: 1156278664
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)