BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0612300 Os12g0612300|AK070162
         (579 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G34730.1  | chr2:14654611-14657443 FORWARD LENGTH=826          174   8e-44
AT5G14990.1  | chr5:4850764-4853003 FORWARD LENGTH=667             63   4e-10

>AT2G34730.1 | chr2:14654611-14657443 FORWARD LENGTH=826
          Length = 825

 Score =  174 bits (442), Expect = 8e-44,   Method: Compositional matrix adjust.
 Identities = 184/638 (28%), Positives = 309/638 (48%), Gaps = 88/638 (13%)

Query: 8   EYELQKEVSNIMIQSYITSMRREFETKLWENQNCISTLNKNWKEKISKIAALRDELSTIY 67
           E++ QKE+ + ++ S + S++ E+E +L + +             I +I  LR EL  I 
Sbjct: 204 EHDFQKEIESAVVTSVLRSLKDEYEQRLLDQKAEFGGNRSLILGNIKEITGLRQELEAIR 263

Query: 68  SVVSASESGVFSSH-GSHEKVEELNFLKMKD--DNESSITERTTDSGELMFDIPDFS-LL 123
                 E+G  +   G  ++VE+L+  KM    ++ SS+ E           IP+ +  L
Sbjct: 264 KSFLDHENGDEAGEVGDRKRVEQLHR-KMSGSLNSVSSVWENGKHEESSTGLIPEHNETL 322

Query: 124 KHMPSEEVTNFLKSEWLKLRRQHESELHEKTEELFRLKREF---AKDIALLPSRKERELE 180
           +HM  +E+ N  K E  K++R H+ ++ E TE+ F  KR++    +  +     K++EL 
Sbjct: 323 RHMSPDEMINHFKIEMNKMKRDHDYKIQELTEQCFTFKRKYLNLTERGSFSFVGKDKELG 382

Query: 181 IIKSKLLQSISKLDEITLREDNPYCDNYDDAEVCGFEDRIGSLLHENEQLQGLLADKKMV 240
            +K K+   ISKLD+I + ++    +  +DA   G + ++ SLL EN QL+  L+D    
Sbjct: 383 ALKKKIPFVISKLDKILMEDEKFVSEGKNDA---GLKRQLDSLLLENRQLKDSLSD---- 435

Query: 241 AKHLSLQVLDAERKMAQHSLSELKLVKQVEKLSHELEDLKIESHI-KDL----------- 288
                     A  KM+Q S +E    + + KL  ++ED + E+ I +D+           
Sbjct: 436 ----------AAEKMSQLSQAEADHQELIRKLETDVEDSRNEASIYEDVYGCFVTEFVGQ 485

Query: 289 ---------LELSTLREVFD-------NYENHIDDANQEETFLRELLIEKEEQLSIMY-- 330
                    LE S LRE ++         E      + E++ ++ +++E  E  S++Y  
Sbjct: 486 IKCTKQETDLEHSMLREAYELLLEDLARKEARKSKEDFEDSCVKSVMME--ECCSVIYKE 543

Query: 331 ---EDRQKLKYENNQLVAIAGSTLMQHHEQVNLVNDLTNFREKVCEQELLILXXXXXXXX 387
              E  +K+   N  +    G+   +  ++  L  ++      V E+E L+         
Sbjct: 544 AVKEAHKKIVELNLHVTEKEGTLRSEMVDKERLKEEIHRLGCLVKEKENLVQTAENNLAT 603

Query: 388 XXXXLYEALQQIN-----VCKQEIHGLTDNLTAMSIA----LEEAKEQNASLDA------ 432
               +    QQIN     V +QE   + D + A+S+     LE+ K     + +      
Sbjct: 604 ERKKIEVVSQQINDLQSQVERQETE-IQDKIEALSVVSARELEKVKGYETKISSLREELE 662

Query: 433 ----TIQEMK---KTSAPSINSHKGQAGHLEYALVSME-----KLSKSYSDFESRLAQSM 480
               +++EMK   + +   ++  K +   L+  LVS++     +L K +   E  +A+  
Sbjct: 663 LARESLKEMKDEKRKTEEKLSETKAEKETLKKQLVSLDLVVPPQLIKGFDILEGLIAEKT 722

Query: 481 KRNEIRLTNIICQFNPLVQQVAVLKKKEFWYKQILEIKCSNLQKAEAEVDILGDEVDALL 540
           ++   RL N+  Q + L  Q+  +K K   YKQ LE KC +L+KAEAEVD+LGDEV+ LL
Sbjct: 723 QKTNSRLKNMQSQLSDLSHQINEVKGKASTYKQRLEKKCCDLKKAEAEVDLLGDEVETLL 782

Query: 541 SILGKIYIALDHYSPVLKHYPGVTEILNLVQKALKGES 578
            +L KIYIALDHYSP+LKHYPG+ EIL LV++ L GES
Sbjct: 783 DLLEKIYIALDHYSPILKHYPGIIEILRLVRRELSGES 820



>AT5G14990.1 | chr5:4850764-4853003 FORWARD LENGTH=667
          Length = 666

 Score = 63.2 bits (152), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 33/109 (30%), Positives = 62/109 (56%)

Query: 469 YSDFESRLAQSMKRNEIRLTNIICQFNPLVQQVAVLKKKEFWYKQILEIKCSNLQKAEAE 528
           Y DF+ ++ + +K    RL N+  + +  +  +A L+++E  Y+    ++  NL+KAE E
Sbjct: 556 YDDFDFKIQEKLKMVTFRLQNLEIKIDSTMDFIAELRQRESVYRTAFVLRSENLRKAETE 615

Query: 529 VDILGDEVDALLSILGKIYIALDHYSPVLKHYPGVTEILNLVQKALKGE 577
           VD+LGD+VD+L+ +L K       +  +L +   + EI  +++K L  E
Sbjct: 616 VDLLGDQVDSLVKLLQKTLWTFHQHPLLLCNNSDILEISKMIKKELLSE 664



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.313    0.130    0.350 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,437,895
Number of extensions: 472409
Number of successful extensions: 1844
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 1835
Number of HSP's successfully gapped: 6
Length of query: 579
Length of database: 11,106,569
Length adjustment: 104
Effective length of query: 475
Effective length of database: 8,255,305
Effective search space: 3921269875
Effective search space used: 3921269875
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 114 (48.5 bits)