BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0596900 Os12g0596900|AK100450
         (289 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G46850.2  | chr5:19010714-19012010 FORWARD LENGTH=326          156   2e-38

>AT5G46850.2 | chr5:19010714-19012010 FORWARD LENGTH=326
          Length = 325

 Score =  156 bits (394), Expect = 2e-38,   Method: Compositional matrix adjust.
 Identities = 89/246 (36%), Positives = 130/246 (52%), Gaps = 10/246 (4%)

Query: 46  SRRYVADSYLDTLIG-QLAQHRDLTETSDSADSCKGLDYDLDAPMLTELHSELVGEGSHR 104
           S +Y+ +SY  T      ++ R+       +D     D ++        H  + GEGSHR
Sbjct: 72  SDQYIMESYFTTYTSLDDSEFRNFLLLQQCSDKVPTQDSNIVPATSFIQHRNITGEGSHR 131

Query: 105 RLVYHVEFANCADAMVNFLDNYDANLVVIENLPNGVFADPFELQHFVERKVYLDMVVFGD 164
            L+  ++       +++  ++    LV++E LP GVFADPFELQ   +R+ + D+ VFGD
Sbjct: 132 NLITSIKL------LLHHSESQFRELVIVERLPLGVFADPFELQSLQQRRAFSDVSVFGD 185

Query: 165 TNLELPSALSNRSSVEIHVDLAPLASMNCNLVIDLPLHARYPPLDASGYATVEFGSPDLL 224
           TNLELPS  SNRS VEIHV+++     N  +   LPLHARY PLD SGY+ VEFG PDL 
Sbjct: 186 TNLELPSFRSNRSVVEIHVEISSRNYENGEISFKLPLHARYQPLDDSGYSRVEFGEPDLF 245

Query: 225 L---RYRKKDTVPESCSWLLKDLDAAPVEKATWRIPCGDEAHIGFVSSITFLSALVCSIL 281
           L     + +      C  L             W IP G   H  +VS++TF +A+  +  
Sbjct: 246 LCSSHVQNQRRERRRCLVLSIGRSKTETRSVFWDIPAGIRGHTEYVSALTFAAAVFSAFS 305

Query: 282 IVIAAL 287
           I++A++
Sbjct: 306 ILVASV 311



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,707,566
Number of extensions: 242535
Number of successful extensions: 487
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 485
Number of HSP's successfully gapped: 1
Length of query: 289
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 191
Effective length of database: 8,419,801
Effective search space: 1608181991
Effective search space used: 1608181991
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 111 (47.4 bits)