BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0576300 Os12g0576300|AK058735
         (411 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G61040.1  | chr5:24562431-24564395 REVERSE LENGTH=591           94   1e-19
AT5G08010.1  | chr5:2569606-2571613 FORWARD LENGTH=567             64   1e-10

>AT5G61040.1 | chr5:24562431-24564395 REVERSE LENGTH=591
          Length = 590

 Score = 94.0 bits (232), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 70/189 (37%), Positives = 102/189 (53%), Gaps = 18/189 (9%)

Query: 65  LSNKNELDALKGTVKRMENLIQDLHDELEMREGLTVKELPNEMSVKIDDDESKAHVTDSE 124
           ++++ E+  +K  +K+ ENL+ DL DELEM++ L VKE+         D E  A    SE
Sbjct: 252 MASQAEVSKVKQELKQTENLVHDLEDELEMKDTLIVKEI---------DIEKAAE--SSE 300

Query: 125 PMSKIXXXXXXXXXXXXXNITSNCLKEQTFDFSEVEQDLIGDIVQGELKIDTTHCDLADH 184
            +S I             N+ S+ ++ +  D  E+E D   +  QGEL+ D       D 
Sbjct: 301 SISNIEAELEAELERLEINMNSSNIETRLSDIIEMEPDCEVEFAQGELRADRVKGKRLD- 359

Query: 185 SSESAHGRD-SRESSPDYTHDANYPVSPRDLSLRLHKVIQQRLEERIKELETTLAQSEMQ 243
             E+   +D S  S+P+     NY VSPR+LSLRLHKVI  RLE+RI ELET L +S+ +
Sbjct: 360 --ETESNQDPSGNSTPE---SGNYAVSPRELSLRLHKVINSRLEKRIGELETALQESQRK 414

Query: 244 TQVQVMATE 252
            +  VM +E
Sbjct: 415 VEQLVMESE 423



>AT5G08010.1 | chr5:2569606-2571613 FORWARD LENGTH=567
          Length = 566

 Score = 63.9 bits (154), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 57/189 (30%), Positives = 92/189 (48%), Gaps = 21/189 (11%)

Query: 65  LSNKNELDALKGTVKRMENLIQDLHDELEMREGLTVKELPNEMSVKIDDDESKAHVTDSE 124
           ++N+ EL+ ++   K+ ENL ++L D++   E                 DE  A   +SE
Sbjct: 230 VANQTELNKVRAESKQTENLGKELEDDIHDGEKQC--------------DEKTAE--NSE 273

Query: 125 PMSKIXXXXXXXXXXXXXNITSNCLKEQTFDFSEVEQDLIGDIVQGELKIDTTHCDLADH 184
            +SKI             N+ S+ ++ +  D  E+E D   +  QGEL+ D       D 
Sbjct: 274 SISKIEAELEAELERLEINMISSNIETKLSDVFELEPDFEVEFAQGELRDDQVERQRFDE 333

Query: 185 SSESAHGRDSRESSPDYTHDANYPVSPRDLSLRLHKVIQQRLEERIKELETTLAQSEMQT 244
           +   ++   S  S+P+     NY VSPR+LSLRL  VI    E+RIKELE  L +S+ + 
Sbjct: 334 TV--SNQERSSNSTPE---SGNYIVSPRELSLRLLGVINSCYEKRIKELENALQESQRKV 388

Query: 245 QVQVMATEQ 253
           +  V+ +E+
Sbjct: 389 EQLVIESEE 397



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.311    0.128    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,431,280
Number of extensions: 260970
Number of successful extensions: 725
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 722
Number of HSP's successfully gapped: 2
Length of query: 411
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 310
Effective length of database: 8,337,553
Effective search space: 2584641430
Effective search space used: 2584641430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 113 (48.1 bits)