BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0575000 Os12g0575000|AK104945
         (190 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G08050.1  | chr5:2578418-2578964 FORWARD LENGTH=159             95   2e-20
>AT5G08050.1 | chr5:2578418-2578964 FORWARD LENGTH=159
          Length = 158

 Score = 94.7 bits (234), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 59/121 (48%), Positives = 68/121 (56%), Gaps = 1/121 (0%)

Query: 59  RQTTSGKAAA-PPVKLLTNVEKLRLLTKAEKAGLLSAAERAGLSLSAVERLGLLSKAEEL 117
           R  TS K+ A P VKLLT VE+L+LLTKAEKAGLLS AE++G SLS +ERLGLL+KAEE 
Sbjct: 27  RTITSKKSTATPQVKLLTRVEQLKLLTKAEKAGLLSLAEKSGFSLSTIERLGLLTKAEEF 86

Query: 118 EVLSAATDXXXXXXXXXXXXXXXXXXXXXXXXXXEEYPWEXXXXXXXXXXXXXGGSTAFA 177
            VLSAAT+                          E+Y WE             GGS AFA
Sbjct: 87  GVLSAATNPETPGTLFTLSLGLLLLGPVFAYVVPEDYTWEVVIQVLVALLSVLGGSAAFA 146

Query: 178 A 178
           A
Sbjct: 147 A 147
  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.310    0.123    0.335 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,391,777
Number of extensions: 76633
Number of successful extensions: 233
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 228
Number of HSP's successfully gapped: 2
Length of query: 190
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 97
Effective length of database: 8,556,881
Effective search space: 830017457
Effective search space used: 830017457
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 109 (46.6 bits)