BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0540700 Os12g0540700|AK107996
         (115 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G21400.1  | chr3:7534824-7536293 REVERSE LENGTH=189            121   6e-29

>AT3G21400.1 | chr3:7534824-7536293 REVERSE LENGTH=189
          Length = 188

 Score =  121 bits (304), Expect = 6e-29,   Method: Compositional matrix adjust.
 Identities = 60/115 (52%), Positives = 79/115 (68%)

Query: 1   MLKHMVGRITTKPGGKPEMGEAFVVDQYNRPLPRVRTSRPEPGEGGHRQLPPGTISVAHV 60
           ML  MVGRI  KPGGK EMGEA VV+   RPLP++R + PE        +P GT++VA V
Sbjct: 73  MLNQMVGRIKAKPGGKAEMGEASVVETSKRPLPKLRNTTPESTRYEENPVPQGTLNVAQV 132

Query: 61  HEIIQLYQGKSSNHPGPMSVDKIASKFRVEASVVQNIVQFVSLPQEEHVTKKEEY 115
             I+ L+QGKS +H GPM V++IA K+R++ S VQ I QF+SLPQE    +K++Y
Sbjct: 133 RHIMLLFQGKSQDHHGPMGVNEIAEKYRIDVSQVQKITQFLSLPQEITDKQKKQY 187



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.133    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,733,654
Number of extensions: 117424
Number of successful extensions: 179
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 179
Number of HSP's successfully gapped: 1
Length of query: 115
Length of database: 11,106,569
Length adjustment: 84
Effective length of query: 31
Effective length of database: 8,803,625
Effective search space: 272912375
Effective search space used: 272912375
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 104 (44.7 bits)