BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0534000 Os12g0534000|AK058923
         (148 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G06350.1  | chr3:1924536-1927701 REVERSE LENGTH=604            177   2e-45

>AT3G06350.1 | chr3:1924536-1927701 REVERSE LENGTH=604
          Length = 603

 Score =  177 bits (449), Expect = 2e-45,   Method: Compositional matrix adjust.
 Identities = 87/130 (66%), Positives = 103/130 (79%)

Query: 14  GAKEKGARVVIANRTFARAQELGNLLGAPALTLAELENYHPEEEMILANTTAIGMHPNVN 73
           GAKEKGA+VVIANRT+ RA EL   +G  AL+L +L+NYHPE+ M+LANTT++GM PNV 
Sbjct: 471 GAKEKGAKVVIANRTYERALELAEAIGGKALSLTDLDNYHPEDGMVLANTTSMGMQPNVE 530

Query: 74  ETPLSKQALKSYAVVFDAVYTPKETRLLREAAECGATVVSGLEMFIRQAMGQFEHFTGMP 133
           ETP+SK ALK YA+VFDAVYTP+ TRLLREA E GA  VSG EMF+RQA  QFE FTG+P
Sbjct: 531 ETPISKDALKHYALVFDAVYTPRITRLLREAEESGAITVSGSEMFVRQAYEQFEIFTGLP 590

Query: 134 APDSLMRDIV 143
           AP  L   I+
Sbjct: 591 APKELYWQIM 600



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,769,157
Number of extensions: 95721
Number of successful extensions: 225
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 225
Number of HSP's successfully gapped: 1
Length of query: 148
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 59
Effective length of database: 8,666,545
Effective search space: 511326155
Effective search space used: 511326155
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 107 (45.8 bits)