BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0514400 Os12g0514400|J023084E14
         (218 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G48160.1  | chr5:19528019-19529820 REVERSE LENGTH=575          234   2e-62
AT3G07780.1  | chr3:2485104-2486876 FORWARD LENGTH=567            182   2e-46
AT3G63500.2  | chr3:23446331-23449991 REVERSE LENGTH=1163          65   3e-11

>AT5G48160.1 | chr5:19528019-19529820 REVERSE LENGTH=575
          Length = 574

 Score =  234 bits (598), Expect = 2e-62,   Method: Compositional matrix adjust.
 Identities = 124/197 (62%), Positives = 154/197 (78%), Gaps = 1/197 (0%)

Query: 19  ELELDSPKSTENEEIGRLITPQEACNRIAEVVHEAVKKMELVAEEKMRLYKKARIAVEAC 78
           E+E DS KS EN E GRL+ PQ+ACNRIAEVV E ++KME+VAEEKMR++KKAR+A+E C
Sbjct: 366 EIESDSAKSFENGEGGRLMAPQDACNRIAEVVQETLRKMEIVAEEKMRMFKKARMALETC 425

Query: 79  DRELDEKARELQELKAERLRKKQQVDELESIVRLKQAEAEMFQLKANEARQEADRLQSIA 138
           DREL++KA+E+ ELKAER +KK Q+DELE IVRLKQAEA+MFQLKANEA++EADRLQ I 
Sbjct: 426 DRELEDKAKEVSELKAERQKKKLQIDELERIVRLKQAEADMFQLKANEAKREADRLQRIV 485

Query: 139 LAKSERAEQDYASLYLKRRLEEAEAEKQFLFEKIKYQETQRPMQXXXXXXXXXXQTPMLS 198
           LAK +++E++YAS YLK+RL EAEAEKQ+LFEKIK QE  R             Q  M S
Sbjct: 486 LAKMDKSEEEYASNYLKQRLSEAEAEKQYLFEKIKLQENSRVASQSSGGGGDPSQVMMYS 545

Query: 199 KIHDLLKNVRSIPPKTE 215
           KI DLL+   ++ PK +
Sbjct: 546 KIRDLLQGY-NLSPKVD 561



>AT3G07780.1 | chr3:2485104-2486876 FORWARD LENGTH=567
          Length = 566

 Score =  182 bits (461), Expect = 2e-46,   Method: Compositional matrix adjust.
 Identities = 111/197 (56%), Positives = 145/197 (73%), Gaps = 7/197 (3%)

Query: 19  ELELDSPKSTENEEIGRLITPQEACNRIAEVVHEAVKKMELVAEEKMRLYKKARIAVEAC 78
           E+ELDSPKS E+ E G  I PQ+ACNRIAEVV E ++KME+V EEK R+YKKAR+ +E C
Sbjct: 364 EIELDSPKSLESGEGGGTIAPQDACNRIAEVVKETLRKMEIVGEEKTRMYKKARMGLEEC 423

Query: 79  DRELDEKARELQELKAERLRKKQQVDELESIVRLKQAEAEMFQLKANEARQEADRLQSIA 138
           +RE++EKA+++ EL+ ER +KKQQ++E+E IVRLKQAEAEMFQLKANEA+ EA+RL+ I 
Sbjct: 424 EREVEEKAKQVAELQMERQKKKQQIEEVERIVRLKQAEAEMFQLKANEAKVEAERLERIV 483

Query: 139 LAKSERAEQDYASLYLKRRLEEAEAEKQFLFEKIKYQETQRPMQXXXXXXXXXXQTPMLS 198
            AK E+ E++YAS YLK RL EAEAEK++LFEKIK QE+               Q  M S
Sbjct: 484 KAKKEKTEEEYASNYLKLRLSEAEAEKEYLFEKIKEQESG-------GNGGEASQAVMYS 536

Query: 199 KIHDLLKNVRSIPPKTE 215
           KI ++L    +  P+ +
Sbjct: 537 KIREMLHGYNASSPRVD 553



>AT3G63500.2 | chr3:23446331-23449991 REVERSE LENGTH=1163
          Length = 1162

 Score = 64.7 bits (156), Expect = 3e-11,   Method: Composition-based stats.
 Identities = 36/73 (49%), Positives = 55/73 (75%), Gaps = 1/73 (1%)

Query: 91   ELKAERLRKKQ-QVDELESIVRLKQAEAEMFQLKANEARQEADRLQSIALAKSERAEQDY 149
            E++ ER   K+ + +ELESIVR+KQAEAEMFQ +A++AR+EA+ L+ IA+AK E+ E++Y
Sbjct: 1043 EMELERGSPKEPRFEELESIVRMKQAEAEMFQGRADDARREAEGLKRIAIAKKEKIEEEY 1102

Query: 150  ASLYLKRRLEEAE 162
                 K  +E+A+
Sbjct: 1103 NRRMGKLSMEDAQ 1115



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.129    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,788,711
Number of extensions: 133704
Number of successful extensions: 1336
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 1359
Number of HSP's successfully gapped: 5
Length of query: 218
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 123
Effective length of database: 8,502,049
Effective search space: 1045752027
Effective search space used: 1045752027
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 109 (46.6 bits)