BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0507000 Os12g0507000|AK065402
         (399 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G31955.1  | chr2:13584635-13586484 REVERSE LENGTH=391          584   e-167

>AT2G31955.1 | chr2:13584635-13586484 REVERSE LENGTH=391
          Length = 390

 Score =  584 bits (1505), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 271/350 (77%), Positives = 310/350 (88%), Gaps = 4/350 (1%)

Query: 50  FSNTYATSCANLPEVAPSEAPLSDMLVDSFGRFHNYLRISLTERCNLRCQYCMPAEGVEL 109
           FS++YA    +       + P+SDML+D FGR H YLRISLTERCNLRCQYCMP+EGVEL
Sbjct: 45  FSSSYAAHQVD----QIKDNPVSDMLIDKFGRLHTYLRISLTERCNLRCQYCMPSEGVEL 100

Query: 110 TPSSELLSHDEIIRVADLFVTSGVDKIRLTGGEPTIRKDIEDICLHLSGLKGLKTLAMTT 169
           TP  +LLS  EI+R+A LFV++GV+KIRLTGGEPT+RKDIE+ICL LS LKGLK LA+TT
Sbjct: 101 TPKPQLLSQSEIVRLAGLFVSAGVNKIRLTGGEPTVRKDIEEICLQLSSLKGLKNLAITT 160

Query: 170 NGLVLSKKLPRLKECGLNALNISLDTLVPAKFEFMTRRKGHSRVMESIDAAIELGFQSVK 229
           NG+ L+KKLPRLKECGL++LNISLDTLVPAKFEF+TRRKGH RVM+SID AIELG+  VK
Sbjct: 161 NGITLAKKLPRLKECGLDSLNISLDTLVPAKFEFLTRRKGHDRVMKSIDTAIELGYNPVK 220

Query: 230 VNCVVMRGMNDDEICDFVEMTRDKPVNVRFIEFMPFDGNVWNVKKLVPYAEIMDKVRQRF 289
           VNCV+MRG+NDDEICDFVE+TRDKP+NVRFIEFMPFDGNVWNVKKLVPYAE+MDKV +RF
Sbjct: 221 VNCVIMRGLNDDEICDFVELTRDKPINVRFIEFMPFDGNVWNVKKLVPYAEVMDKVVKRF 280

Query: 290 NGVERLQDHPSETAKNFKIDGHAGTISFITSMTQHFCAGCNRLRLLADGNLKVCLFGPSE 349
             ++R+QDHP+ETAKNF IDGH G++SFITSMT+HFCAGCNRLRLLADGN KVCLFGPSE
Sbjct: 281 PSIKRMQDHPTETAKNFTIDGHCGSVSFITSMTEHFCAGCNRLRLLADGNFKVCLFGPSE 340

Query: 350 VSLREPIRAGVDDAGLREIISAAVKRKKAKHAGMFDIAKTANRPMIHIGG 399
           VSLR+P+R+G DD  LREII AAVKRKKA HAGM DIAKTANRPMIHIGG
Sbjct: 341 VSLRDPLRSGADDEALREIIGAAVKRKKAAHAGMLDIAKTANRPMIHIGG 390



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.137    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,419,676
Number of extensions: 344986
Number of successful extensions: 807
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 807
Number of HSP's successfully gapped: 1
Length of query: 399
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 298
Effective length of database: 8,337,553
Effective search space: 2484590794
Effective search space used: 2484590794
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 113 (48.1 bits)