BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0500700 Os12g0500700|AK073408
         (306 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G53530.1  | chr5:21746275-21748156 REVERSE LENGTH=303          496   e-141
AT4G27690.1  | chr4:13824019-13826235 FORWARD LENGTH=304          493   e-140

>AT5G53530.1 | chr5:21746275-21748156 REVERSE LENGTH=303
          Length = 302

 Score =  496 bits (1276), Expect = e-141,   Method: Compositional matrix adjust.
 Identities = 231/301 (76%), Positives = 274/301 (91%), Gaps = 2/301 (0%)

Query: 1   MNYIVGAFKPPCDISIAFSDARSRKQIAVKNDNGRTVMVPVFQSLETISGEVSVVPVPGK 60
           MNY++GAFKP C+ISI F+D ++RKQ+  K DNG+ VM P+FQS ETI+G++++ P  GK
Sbjct: 1   MNYLLGAFKPACNISITFTDGKNRKQVPTKKDNGQIVMNPLFQSQETIAGKINIEPYQGK 60

Query: 61  RIEHLGVKIELLGQIELYLERGKFYDFTSLVRELDIPGEIYEKKTYPFEFSTVEMPYESY 120
           ++EH GVK+ELLGQIE+Y +RG FYDFTSLVRE+D+PGEIYE+KTYPFEFS+VEMPYE+Y
Sbjct: 61  KVEHNGVKVELLGQIEMYFDRGNFYDFTSLVREIDVPGEIYERKTYPFEFSSVEMPYETY 120

Query: 121 NGTNVRLRYILKVTISRNIMGMGSIVEFREFCVRNYSPAPTINNSIKMEVGIEDCLHIEF 180
           NG NVRLRY+LKVT++R   G  SIVE+++F VRNY P P INNSIKMEVGIEDCLHIEF
Sbjct: 121 NGVNVRLRYVLKVTVTRGYAG--SIVEYQDFVVRNYVPLPPINNSIKMEVGIEDCLHIEF 178

Query: 181 EYSKSKYHLKDVIIGKIYFLLVRIKIKNMELEVRRRESTGAGSSAYVETETLAKFELMDG 240
           EY+KSKYHLKDVI+GKIYFLLVRIKIKNM+LE+RRRESTGAG++ +VETETLAKFELMDG
Sbjct: 179 EYNKSKYHLKDVILGKIYFLLVRIKIKNMDLEIRRRESTGAGANTHVETETLAKFELMDG 238

Query: 241 APVRGESIPVRLFLTPYELTPSYRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITMYRLL 300
           APVRGESIPVR+FLTPY+LTP+++NINNKFSVKYYLNLVLVDEEDRRYFKQQEIT+YRL 
Sbjct: 239 APVRGESIPVRVFLTPYDLTPTHKNINNKFSVKYYLNLVLVDEEDRRYFKQQEITLYRLK 298

Query: 301 E 301
           E
Sbjct: 299 E 299



>AT4G27690.1 | chr4:13824019-13826235 FORWARD LENGTH=304
          Length = 303

 Score =  493 bits (1268), Expect = e-140,   Method: Compositional matrix adjust.
 Identities = 230/305 (75%), Positives = 272/305 (89%), Gaps = 2/305 (0%)

Query: 1   MNYIVGAFKPPCDISIAFSDARSRKQIAVKNDNGRTVMVPVFQSLETISGEVSVVPVPGK 60
           MNY++GAFKP C+ISI FSD ++RKQ+ +K +NG+T +VP+F S +TISG+V + P  GK
Sbjct: 1   MNYLLGAFKPACNISITFSDGKNRKQVPMKKENGQTALVPLFHSQDTISGKVCIEPYQGK 60

Query: 61  RIEHLGVKIELLGQIELYLERGKFYDFTSLVRELDIPGEIYEKKTYPFEFSTVEMPYESY 120
           ++EH GVK+ELLGQIE+Y +RG FYDFTSLVRELD+PGEIYE+KTYPFEF TVEMPYE+Y
Sbjct: 61  KVEHNGVKVELLGQIEMYFDRGNFYDFTSLVRELDVPGEIYERKTYPFEFPTVEMPYETY 120

Query: 121 NGTNVRLRYILKVTISRNIMGMGSIVEFREFCVRNYSPAPTINNSIKMEVGIEDCLHIEF 180
           NG NVRLRY+LKVT++R   G  SI+E++E  VRNY+P P INNSIKMEVGIEDCLHIEF
Sbjct: 121 NGVNVRLRYVLKVTVTRGYAG--SILEYQELVVRNYAPLPDINNSIKMEVGIEDCLHIEF 178

Query: 181 EYSKSKYHLKDVIIGKIYFLLVRIKIKNMELEVRRRESTGAGSSAYVETETLAKFELMDG 240
           EY+KSKYHLKDVI+GKIYFLLVRIK+KNM+LE+RRRESTGAG++ +VETETLAKFELMDG
Sbjct: 179 EYNKSKYHLKDVILGKIYFLLVRIKMKNMDLEIRRRESTGAGANTHVETETLAKFELMDG 238

Query: 241 APVRGESIPVRLFLTPYELTPSYRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITMYRLL 300
            PVRGESIPVRLFL PY+LTP++RNINNKFSVKYYLNLVLVDEEDRRYFKQQEIT+YRL 
Sbjct: 239 TPVRGESIPVRLFLAPYDLTPTHRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITLYRLK 298

Query: 301 ETPQA 305
           E   +
Sbjct: 299 EDASS 303



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.139    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,548,672
Number of extensions: 277566
Number of successful extensions: 540
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 538
Number of HSP's successfully gapped: 2
Length of query: 306
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 208
Effective length of database: 8,419,801
Effective search space: 1751318608
Effective search space used: 1751318608
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)