BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0482500 Os12g0482500|AK120590
         (424 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G16720.1  | chr5:5494309-5496562 FORWARD LENGTH=676             51   1e-06
AT1G70750.1  | chr1:26680728-26683147 REVERSE LENGTH=750           49   4e-06

>AT5G16720.1 | chr5:5494309-5496562 FORWARD LENGTH=676
          Length = 675

 Score = 50.8 bits (120), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 24/52 (46%), Positives = 36/52 (69%)

Query: 241 EAAEYQRQLEEQGEFDREAVRLAMQLVHEAETEKHALQRQLDAFRVKAHLYD 292
           EA +YQR +EEQ E+D+EA++L   L+ + E EK  LQR+L+ +R K   Y+
Sbjct: 409 EALQYQRMMEEQAEYDQEALQLLNHLMVKREKEKEQLQRELEVYRAKVLEYE 460



>AT1G70750.1 | chr1:26680728-26683147 REVERSE LENGTH=750
          Length = 749

 Score = 49.3 bits (116), Expect = 4e-06,   Method: Compositional matrix adjust.
 Identities = 39/135 (28%), Positives = 58/135 (42%), Gaps = 26/135 (19%)

Query: 184 EDGHGNAHEVFDDLPAELRRDG----DGALTVGXXXXXXXXXXXELDAVRAELDGERRAC 239
           E  H + H     L   +  DG    +G LTV             L A+  EL+ ER A 
Sbjct: 382 ESSHSSLHNAMFHLEQRVSVDGIECPEGVLTVDKLKFELQEERKALHALYEELEVERNAS 441

Query: 240 A----------------------EAAEYQRQLEEQGEFDREAVRLAMQLVHEAETEKHAL 277
           A                      EA +YQR +EEQ EFD+EA++L  +L+   E E   L
Sbjct: 442 AVAASETMAMINRLHEEKAAMQMEALQYQRMMEEQAEFDQEALQLLNELMVNREKENAEL 501

Query: 278 QRQLDAFRVKAHLYD 292
           +++L+ +R +   Y+
Sbjct: 502 EKELEVYRKRMEEYE 516



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,938,016
Number of extensions: 242006
Number of successful extensions: 570
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 568
Number of HSP's successfully gapped: 2
Length of query: 424
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 323
Effective length of database: 8,337,553
Effective search space: 2693029619
Effective search space used: 2693029619
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 113 (48.1 bits)