BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0456200 Os12g0456200|AK069528
         (260 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G06410.1  | chr5:1959719-1961132 REVERSE LENGTH=253            259   1e-69

>AT5G06410.1 | chr5:1959719-1961132 REVERSE LENGTH=253
          Length = 252

 Score =  259 bits (661), Expect = 1e-69,   Method: Compositional matrix adjust.
 Identities = 119/186 (63%), Positives = 153/186 (82%)

Query: 75  CWSCGASGAFLSCGSCGSVQPVDPAVDYFRIFGLEREYTIKDNNLEGKYKDWQKKLHPDL 134
           CW+CG   AFL C SC S+QPVD +VDYF+IFGLE++Y I   +LEGKYKDWQKKLHPDL
Sbjct: 67  CWNCGEKAAFLFCNSCRSIQPVDDSVDYFQIFGLEKKYEIDPGSLEGKYKDWQKKLHPDL 126

Query: 135 VHSKSEKERAFAAEQSALVIDAYRTLSKPLSRALYLLQLEGIPIDEEKTITDPELLMEMM 194
           VH+KS+KER +AAEQSA V +A RTL+K LSRA+Y+++L G+ ++EE+TITDP LLME+M
Sbjct: 127 VHNKSKKERDYAAEQSAKVTEACRTLTKRLSRAMYIMKLNGVNVNEEETITDPTLLMEIM 186

Query: 195 EIREAVNDANDSQTLEKIQSQIKRKLETWSHSFQEAFERRDFDRAVKATQRMRYYERAVE 254
           E+REA+++A+DS +L +I+SQ++ KL+ WS SF EAFE + FD AVK  QRM YYERA E
Sbjct: 187 ELREAISEADDSTSLNQIRSQVQEKLKQWSDSFVEAFESQKFDDAVKCIQRMTYYERACE 246

Query: 255 ETIKKL 260
           E +KKL
Sbjct: 247 EILKKL 252



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.133    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,151,938
Number of extensions: 151128
Number of successful extensions: 628
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 630
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 163
Effective length of database: 8,447,217
Effective search space: 1376896371
Effective search space used: 1376896371
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)