BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0283400 Os12g0283400|AK110798
         (228 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G64620.1  | chr5:25831875-25832417 FORWARD LENGTH=181           82   2e-16

>AT5G64620.1 | chr5:25831875-25832417 FORWARD LENGTH=181
          Length = 180

 Score = 82.0 bits (201), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 47/157 (29%), Positives = 70/157 (44%), Gaps = 11/157 (7%)

Query: 64  LVRATCNSTSYYDLCVAELSADPSSATADVRGLXXXXXXXXXXXXXXXXXXXXXXXXXXX 123
           ++ +TC +T+YY  CV+ L +DP S TAD +GL                           
Sbjct: 30  IIESTCKTTNYYKFCVSALKSDPRSPTADTKGLASIMVGVGMTNATSTANYIAGNLSATV 89

Query: 124 XXXXXXXXXXXXXXXXQRLLATCAAKYGDARDALAAAKGSIAQQDYDMASVHVSAAAEYP 183
                           +++L  C+ KY  A D+L      +  + YD AS+HV AA +YP
Sbjct: 90  KDTVL-----------KKVLQDCSEKYALAADSLRLTIQDLDDEAYDYASMHVLAAQDYP 138

Query: 184 QVCRTLFGRQSPGDYPPELAATEVALRQLCSVALDII 220
            VCR +F R     YP E+   E +LR++C V   I+
Sbjct: 139 NVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGIL 175



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.124    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,637,191
Number of extensions: 59423
Number of successful extensions: 194
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 194
Number of HSP's successfully gapped: 1
Length of query: 228
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 133
Effective length of database: 8,502,049
Effective search space: 1130772517
Effective search space used: 1130772517
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)