BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0225100 Os12g0225100|AK059945
         (243 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G43970.1  | chr2:18205535-18208031 REVERSE LENGTH=546           98   5e-21
AT3G19090.1  | chr3:6601466-6603709 FORWARD LENGTH=456             79   2e-15
AT5G46250.1  | chr5:18755388-18758056 FORWARD LENGTH=423           68   4e-12

>AT2G43970.1 | chr2:18205535-18208031 REVERSE LENGTH=546
          Length = 545

 Score = 97.8 bits (242), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 58/117 (49%), Positives = 81/117 (69%), Gaps = 9/117 (7%)

Query: 1   SVKTIRTCYPQSS-NGNSPATNRSAKLD-MLFANKLHAFVEYETLEDAEKAILELNDEKN 58
           SVK IRTC PQ++ +G  PA   +AK D  LF+NK+HAFVEYE +E AE+A+ EL++  N
Sbjct: 310 SVKNIRTCQPQNNGSGAPPAARSAAKSDGTLFSNKVHAFVEYEIVELAERAVTELSEAGN 369

Query: 59  WRNGLRVRLLNTCMTK----GAGKGKKGVHEADGNGEEDVSTSNQSN--EKQLEEPS 109
           WR+GL+VRL+    TK    G G+G+KG H+AD   EED +T+++    EKQ ++ S
Sbjct: 370 WRSGLKVRLMLKHQTKEPKQGQGRGRKG-HDADVEHEEDDATTSEQQPIEKQSDDCS 425




 Score = 50.1 bits (118), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 25/45 (55%), Positives = 31/45 (68%), Gaps = 3/45 (6%)

Query: 186 VGTPPNQQQTKPEQHPQLPIGATKQPPGPRMPDGTRGFTLGRGKP 230
           VGT P+     P  + + P    +QPPGPRMPDGTRGF++GRGKP
Sbjct: 498 VGTQPS---NNPMNNMEQPGMGKQQPPGPRMPDGTRGFSMGRGKP 539



>AT3G19090.1 | chr3:6601466-6603709 FORWARD LENGTH=456
          Length = 455

 Score = 79.0 bits (193), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 38/80 (47%), Positives = 50/80 (62%), Gaps = 6/80 (7%)

Query: 2   VKTIRTCYPQSSNGNSPATNRSAKLDMLFANKLHAFVEYETLEDAEKAILELNDEKNWRN 61
           VK IR C+P  SN + P      K D L +NK+HA +EY+    A+KA+ +LNDE+NWR 
Sbjct: 262 VKAIRICHPPESNSSRP------KGDFLMSNKIHALIEYDNTVIADKAVEKLNDERNWRK 315

Query: 62  GLRVRLLNTCMTKGAGKGKK 81
           GLRVRLL  C  K   K ++
Sbjct: 316 GLRVRLLLRCSPKSVLKNRR 335



>AT5G46250.1 | chr5:18755388-18758056 FORWARD LENGTH=423
          Length = 422

 Score = 68.2 bits (165), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 41/109 (37%), Positives = 59/109 (54%), Gaps = 7/109 (6%)

Query: 1   SVKTIRTCYPQSSNGNSPATNRSAKLDMLFANKLHAFVEYETLEDAEKAILELNDEKNWR 60
           S+K++  C P +       + +  K +     +LHAFVEYET+E AEKA   LN+E++WR
Sbjct: 218 SIKSVSICDPNAVE----ESEKGGKKENFIRTRLHAFVEYETVEAAEKAAATLNNEQDWR 273

Query: 61  NGLRVRLLNTCMTKGAGK--GKKGV-HEADGNGEEDVSTSNQSNEKQLE 106
           NGLRV+LL     K A +   ++ V  E D  G     T  + N+K  E
Sbjct: 274 NGLRVKLLEQAAGKFAQRRPARREVDKEKDTTGRVHDQTGGEKNKKTRE 322




 Score = 53.9 bits (128), Expect = 8e-08,   Method: Compositional matrix adjust.
 Identities = 23/34 (67%), Positives = 27/34 (79%), Gaps = 1/34 (2%)

Query: 200 HPQL-PIGATKQPPGPRMPDGTRGFTLGRGKPQP 232
           HP   P+  +K+PPGPRMPDGTRGFT+GRGK  P
Sbjct: 378 HPNYHPVEVSKRPPGPRMPDGTRGFTMGRGKAIP 411



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.309    0.130    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,842,668
Number of extensions: 201514
Number of successful extensions: 741
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 739
Number of HSP's successfully gapped: 5
Length of query: 243
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 147
Effective length of database: 8,474,633
Effective search space: 1245771051
Effective search space used: 1245771051
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 110 (47.0 bits)