BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0174800 Os12g0174800|AK064263
         (328 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G71860.1  | chr1:27026866-27028675 FORWARD LENGTH=341          250   8e-67

>AT1G71860.1 | chr1:27026866-27028675 FORWARD LENGTH=341
          Length = 340

 Score =  250 bits (638), Expect = 8e-67,   Method: Compositional matrix adjust.
 Identities = 134/296 (45%), Positives = 175/296 (59%), Gaps = 11/296 (3%)

Query: 37  QVGLCREALEYFEGMCGRPEAMSDEFRRLQDTR---HELMXXXXXXXXXXXXXKNRYIDV 93
           Q+  C +AL  F G    P++++ EF  LQ  R    EL+             KNRY DV
Sbjct: 37  QLNHCHQALGVFRGKIQNPDSIAHEFTGLQANRMWPSELLLNSTVAMNSVNVEKNRYSDV 96

Query: 94  VPFDTTRVRLKRSTTSQTSSNDYINASFIKVTEDNRVAKFISTQGPLAKTFDDFWEMVYE 153
           VPFD  R+ L     S  S+  Y+NAS IK +E   +++FI+TQGPL  T +DFWEMV +
Sbjct: 97  VPFDKNRIVLNPCKDS--SAKGYVNASLIKTSESESISQFIATQGPLPHTMEDFWEMVIQ 154

Query: 154 YQCPVIVMLTQF----DSLKCDEYLPLRKQREAYGKYNVKITNAKRDSHQLWLRDVMVQC 209
             CP+IVMLT+      ++KC +Y         +G  ++     K     L LR++ V  
Sbjct: 155 QHCPIIVMLTRLVDNNRTVKCGDYFQDEDGPREFGNISLTTKWIKTTDTSLMLRNLEVNY 214

Query: 210 NES-SRVHSVRHIEYPDWPDHGVPTNTDAVRQIRKWL-QNTPMEHPIVVHCSAGIGRTGA 267
            E+  +  SV HI+YP+WPDHGVP +T AVR+I K L Q  P   PI+VHCSAGIGRTG 
Sbjct: 215 KETEDQPMSVLHIQYPEWPDHGVPKDTVAVREILKRLYQVPPSLGPIIVHCSAGIGRTGT 274

Query: 268 YITIHSTIERLLLGDKSSYHLDETVKTLRTQRVGMVQTEKQYMFCYRAIADELKDL 323
           Y  IH+TI+R+L GD S+  L +TV   R QR+GMVQT  QY FCY AI DEL+DL
Sbjct: 275 YCAIHNTIQRILAGDMSALDLAKTVALFRKQRIGMVQTMDQYFFCYNAIVDELEDL 330



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.134    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,029,398
Number of extensions: 225830
Number of successful extensions: 481
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 477
Number of HSP's successfully gapped: 1
Length of query: 328
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 229
Effective length of database: 8,392,385
Effective search space: 1921856165
Effective search space used: 1921856165
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)