BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0163300 Os12g0163300|AK069765
         (319 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G58180.1  | chr3:21544189-21545981 FORWARD LENGTH=315          392   e-109
AT3G62530.1  | chr3:23132219-23133121 FORWARD LENGTH=222          114   5e-26

>AT3G58180.1 | chr3:21544189-21545981 FORWARD LENGTH=315
          Length = 314

 Score =  392 bits (1007), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 199/302 (65%), Positives = 234/302 (77%), Gaps = 3/302 (0%)

Query: 18  MEKFLCDLLLDTAQPIAERFRALFSLRNLHGDGPRCALLQAARDSSNLLAHEAAFALGQM 77
           +EKFLC+ L+D +QPI+ERFRALFSLRNL G GPR AL+ A+RDSSNLLAHEAAFALGQM
Sbjct: 13  LEKFLCERLVDQSQPISERFRALFSLRNLKGPGPRNALILASRDSSNLLAHEAAFALGQM 72

Query: 78  QDAEAIPALEAVLKDLSLHPIVRHXXXXXXXXXXXXKSIPLLEESLAADPAVEVQETCEL 137
           QDAEAIPALE+VL D+SLHPIVRH             ++ +L++SL++DPA EV+ETCEL
Sbjct: 73  QDAEAIPALESVLNDMSLHPIVRHEAAEALGAIGLAGNVNILKKSLSSDPAQEVRETCEL 132

Query: 138 ALRRIEQQKNAGV---SESTTISPFLSVDPALPAKQGLSVHQLREILLNEQESMYERYAA 194
           AL+RIE   N      S +T  SPF+SVDPA PA    SVHQLR++LL+E + MYERYAA
Sbjct: 133 ALKRIEDMSNVDAENQSSTTEKSPFMSVDPAGPAASFSSVHQLRQVLLDETKGMYERYAA 192

Query: 195 LFALRNDSRDXXXXXXXXXXXXKSALLKHEVAYVLGQLQNKAASDALSTVLKNVDEHPMV 254
           LFALRN   +             SALL+HEVAYVLGQLQ+K A   LS VL++V+EHPMV
Sbjct: 193 LFALRNHGGEEAVSAIVDSLSASSALLRHEVAYVLGQLQSKTALATLSKVLRDVNEHPMV 252

Query: 255 RHEAAEALGSIADQESIALLEEFAKDPEPIVSQSCEVALSMLEYERSGKSFEFLFLQTPG 314
           RHEAAEALGSIAD++SIALLEEF+KDPEPIV+QSCEVALSMLE+E SGKSFEF F Q P 
Sbjct: 253 RHEAAEALGSIADEQSIALLEEFSKDPEPIVAQSCEVALSMLEFENSGKSFEFFFTQDPL 312

Query: 315 VQ 316
           V 
Sbjct: 313 VH 314



>AT3G62530.1 | chr3:23132219-23133121 FORWARD LENGTH=222
          Length = 221

 Score =  114 bits (286), Expect = 5e-26,   Method: Compositional matrix adjust.
 Identities = 59/131 (45%), Positives = 89/131 (67%)

Query: 173 SVHQLREILLNEQESMYERYAALFALRNDSRDXXXXXXXXXXXXKSALLKHEVAYVLGQL 232
           S+H +RE LL E E  Y R  ALFALRN   +            +S++++ E A+VLGQL
Sbjct: 86  SIHSMRESLLEETEEDYYRRLALFALRNHGGEDAINVIIESLGVESSMIRIEAAFVLGQL 145

Query: 233 QNKAASDALSTVLKNVDEHPMVRHEAAEALGSIADQESIALLEEFAKDPEPIVSQSCEVA 292
           ++K A  +LS +L++V EHPMVR EAA+ALG IAD++S  +L+E + D +PI+++ C+ +
Sbjct: 146 ESKTAIASLSKILRDVKEHPMVRVEAAKALGFIADEKSREVLQELSGDLDPIIAKGCDSS 205

Query: 293 LSMLEYERSGK 303
           LS+LE++ S K
Sbjct: 206 LSILEFKNSKK 216




 Score = 52.0 bits (123), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 37/119 (31%), Positives = 63/119 (52%), Gaps = 2/119 (1%)

Query: 25  LLLDTAQPIAERFRALFSLRNLHGDGPRCALLQAARDSSNLLAHEAAFALGQMQDAEAIP 84
           LL +T +    R  ALF+LRN  G+     ++++    S+++  EAAF LGQ++   AI 
Sbjct: 94  LLEETEEDYYRRL-ALFALRNHGGEDAINVIIESLGVESSMIRIEAAFVLGQLESKTAIA 152

Query: 85  ALEAVLKDLSLHPIVRHXXXXXXXXXXXXKSIPLLEESLAADPAVEVQETCELALRRIE 143
           +L  +L+D+  HP+VR             KS  +L+E L+ D    + + C+ +L  +E
Sbjct: 153 SLSKILRDVKEHPMVRVEAAKALGFIADEKSREVLQE-LSGDLDPIIAKGCDSSLSILE 210



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.129    0.351 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,438,295
Number of extensions: 180980
Number of successful extensions: 527
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 518
Number of HSP's successfully gapped: 3
Length of query: 319
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 220
Effective length of database: 8,392,385
Effective search space: 1846324700
Effective search space used: 1846324700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 112 (47.8 bits)