BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0143300 Os12g0143300|J065138F13
(112 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G43210.1 | chr5:17347567-17348379 FORWARD LENGTH=171 87 1e-18
AT2G30350.2 | chr2:12934237-12935742 REVERSE LENGTH=369 48 9e-07
>AT5G43210.1 | chr5:17347567-17348379 FORWARD LENGTH=171
Length = 170
Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust.
Identities = 51/111 (45%), Positives = 64/111 (57%), Gaps = 2/111 (1%)
Query: 3 LSATFRSAKIPRALPPKSGEPXXXXXXXXXXXPGNPS-AEAAKGKAALGWCVYLIASS-R 60
LS TF S K+ + P P + S E +K + W VYLI S+
Sbjct: 5 LSRTFPSVKLRDSNPKAPEFKNLKNSQTQVPIPSSSSPQENSKIPKSKSWSVYLILSTTE 64
Query: 61 ISRTYVGVTTDFPRRLRQHNGELKGGAKASSAGRPWNLACLIEGFVNRSEG 111
+TYVG+TTDF RRL+QHNGE++GGAKASSAGRPW AC+I GF S+
Sbjct: 65 PIKTYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTCLSQA 115
>AT2G30350.2 | chr2:12934237-12935742 REVERSE LENGTH=369
Length = 368
Score = 48.1 bits (113), Expect = 9e-07, Method: Compositional matrix adjust.
Identities = 20/43 (46%), Positives = 27/43 (62%)
Query: 63 RTYVGVTTDFPRRLRQHNGELKGGAKASSAGRPWNLACLIEGF 105
+TY+G T + RR+RQHNGE+ GA + RPW + I GF
Sbjct: 43 QTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRPWEMVLCIYGF 85
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.316 0.132 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,134,421
Number of extensions: 69602
Number of successful extensions: 115
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 115
Number of HSP's successfully gapped: 2
Length of query: 112
Length of database: 11,106,569
Length adjustment: 81
Effective length of query: 31
Effective length of database: 8,885,873
Effective search space: 275462063
Effective search space used: 275462063
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 104 (44.7 bits)