BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0133400 Os12g0133400|AK071502
         (282 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G11470.2  | chr3:3610261-3612094 REVERSE LENGTH=301             55   3e-08

>AT3G11470.2 | chr3:3610261-3612094 REVERSE LENGTH=301
          Length = 300

 Score = 55.5 bits (132), Expect = 3e-08,   Method: Compositional matrix adjust.
 Identities = 53/219 (24%), Positives = 92/219 (42%), Gaps = 26/219 (11%)

Query: 36  AAVLPPHDRPAIHRFVREEDRKRALVSRLLKYSLVHHALG---MPFHQIRINRTLEGKPY 92
           + +L P ++  + +   +E +K AL++R L  + +   +    +    +   + + GKP 
Sbjct: 51  SQLLSPTEKEKVLQMRGDELKKNALLARTLVRTTIARCMTNNEVDPKALMFKKNMYGKPE 110

Query: 93  LN----KNVNLPGFNFNTSHQGEYVGIASEPLCLVGLDIVCISKPQRETALEFINNFTSY 148
           ++     N N P  +FN SH    +         VG+D+    +  +   L F   F S 
Sbjct: 111 VDWQNYTNCNNPPLHFNISHTDSLIACGVTVHVPVGIDVEDKERKIKHDILAFAERFYSA 170

Query: 149 LTDHEWNCIVTADSHDGMLTEFYRYWCLKEAFVKATGAGVG------FGLQ-----RLEF 197
               E   + T    +    EF + W LKEA+VKA G G        F +Q     + E+
Sbjct: 171 ---DEVKFLSTLPDPEVQRKEFIKLWTLKEAYVKALGKGFSAAPFNTFTIQSKVGTKGEY 227

Query: 198 HHMNWTNISLCIDGEEARK----WMFWLFKIDEMHLASI 232
           +    T ++     EE  K    W F L ++D+ H A+I
Sbjct: 228 NLCKMTEMTAS-SLEETNKCDGEWKFSLLELDDSHYAAI 265



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.138    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,627,149
Number of extensions: 282884
Number of successful extensions: 597
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 597
Number of HSP's successfully gapped: 1
Length of query: 282
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 185
Effective length of database: 8,447,217
Effective search space: 1562735145
Effective search space used: 1562735145
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 111 (47.4 bits)