BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0112300 Os12g0112300|AK068723
         (499 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G05310.3  | chr5:1571271-1574458 FORWARD LENGTH=512            517   e-147

>AT5G05310.3 | chr5:1571271-1574458 FORWARD LENGTH=512
          Length = 511

 Score =  517 bits (1332), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 280/489 (57%), Positives = 340/489 (69%), Gaps = 10/489 (2%)

Query: 10  RLR-DAAVASVVAVLPHEISPLISAASTFFFILSAYFAVLPLRDEGAISLGLSSLPGLFA 68
           R+R DA ++++V V PHE+  L+ ++S FFFILSAYF VLPLRDEGAISLGLS LPGLF 
Sbjct: 4   RIRLDAVISTIVTVHPHELPALLHSSSCFFFILSAYFVVLPLRDEGAISLGLSKLPGLFV 63

Query: 69  GSLLLTLVAAPVASLAFSLPSIPKPRALVFIHRXXXXXXXXXXXXWFASTPGHSPS---- 124
           GSL LTL+AAP+++  FSLP++ K +ALV IHR            W AS P  S S    
Sbjct: 64  GSLFLTLIAAPLSTFIFSLPNLSKSKALVIIHRFFSLSLVLCFLLWMAS-PTESNSKDAV 122

Query: 125 --ISQSSEDASNKPPGWGNHSWFYILVRISLFLWVSLLNLIAISSTWARVIDIMDSESGS 182
              S  + D +N   GW NH WFYI VR+  FLWV+LLNL+AISSTWAR+ID+MD +SG+
Sbjct: 123 EAASGLTNDGANAATGWDNHGWFYISVRVGFFLWVALLNLVAISSTWARIIDVMDIQSGA 182

Query: 183 RLFGFIGAGATLGQLFGSLFAXXXXXXXXXXXXXXXXXXXXXXXXXKGICTDDTHGSMEL 242
           RLFGF+GAGATLGQLFGS+FA                         KGI  D +  S EL
Sbjct: 183 RLFGFVGAGATLGQLFGSVFAAATAWMGPYLLLFAALLMEFAAQSSKGITNDISQSSEEL 242

Query: 243 S--STIAEQSQNTEADDEMSSLVTSQGLPSQVSESQKTKPEIFVMFEGFWLIARSPYLMY 300
           S         Q     +  S  V S  + S  S    T+P+ + + +G  LI  SPYL+ 
Sbjct: 243 SPLRGTDNDHQRERKQEATSPKVGSPKVASPKSPISTTRPQFWAILDGMRLILASPYLLL 302

Query: 301 ISLFLWLSAVVSSFFYFQKVTIVATTISSPTARRRTFALINSFIAVFILAGQLTLTGRIL 360
           +SLFLWL AV+SSFFYFQKV I+ATTI S   RRR FA INSF+AVFIL GQLTLTGRIL
Sbjct: 303 VSLFLWLGAVISSFFYFQKVNIIATTIKSSIGRRRLFAQINSFVAVFILIGQLTLTGRIL 362

Query: 361 TIAGVTVAICASPFIATLNMVALALWPTWVAVAVTETIRKVTTYVLTRPGRELLFTVVSQ 420
           T+AGVTVAI ASPF+A  N+VA+A+WPTWV VAV+ET+RKVTTYV+TRPGRELLFTVVSQ
Sbjct: 363 TVAGVTVAISASPFVALGNLVAIAIWPTWVTVAVSETLRKVTTYVVTRPGRELLFTVVSQ 422

Query: 421 DEKYKAKVCIDVVVQRLGDATAAGIYSLLFSSLEKKASMVTLYALPLCFVWLLTAFHLGR 480
           DEKYKAKVCIDV+VQRLGDA AAG++ +L  +L  + S  +LYALP+C +W++TAF LGR
Sbjct: 423 DEKYKAKVCIDVIVQRLGDAAAAGLFEVLTIALGGQTSTASLYALPVCLIWIVTAFFLGR 482

Query: 481 LQTNLVKVQ 489
            Q  L K+Q
Sbjct: 483 RQEQLAKLQ 491



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.324    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,628,152
Number of extensions: 303073
Number of successful extensions: 1241
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1239
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 396
Effective length of database: 8,282,721
Effective search space: 3279957516
Effective search space used: 3279957516
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 114 (48.5 bits)