BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os11g0580000 Os11g0580000|AK119421
(622 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G54385.1 | chr1:20301289-20303048 REVERSE LENGTH=561 446 e-125
AT3G03970.1 | chr3:1028144-1029891 REVERSE LENGTH=555 280 1e-75
AT2G07170.1 | chr2:2975565-2978692 FORWARD LENGTH=821 49 9e-06
>AT1G54385.1 | chr1:20301289-20303048 REVERSE LENGTH=561
Length = 560
Score = 446 bits (1147), Expect = e-125, Method: Compositional matrix adjust.
Identities = 251/488 (51%), Positives = 321/488 (65%), Gaps = 36/488 (7%)
Query: 1 MGRSLSPLLRQELDNLDKDADSRRNAMKALKSYAKHLDSKSIPHFLAEVSDNKAAPGLPS 60
MG +L+P+LRQEL NLDKD +SR++AMKALKSY K LDSK+IP FLA+V + K L S
Sbjct: 1 MGLNLNPILRQELANLDKDTESRKSAMKALKSYVKDLDSKAIPGFLAQVFETKETNSL-S 59
Query: 61 GEFTISLYEVLARVHGRNIVPQVGNIMSTIMCTLSSSGGSFPLHQACSKVVPAIARYGID 120
GE+TISLYE+LARVHG NIVPQ+ IMSTI+ TL+SS GSFPL QACSKV+PAIARYGID
Sbjct: 60 GEYTISLYEILARVHGPNIVPQIDTIMSTIVKTLASSAGSFPLQQACSKVIPAIARYGID 119
Query: 121 PSTPDEEKAGIIASLCKPLCGALMGNQDGAASGAALCLKALVESSNWRFAAGETVNEVCL 180
P+T +++K II SLCKPL +L+ +Q+ SGAALCLKALV+S NWRFA+ E VN VC
Sbjct: 120 PTTTEDKKRVIIHSLCKPLTDSLLASQESLTSGAALCLKALVDSDNWRFASDEMVNRVCQ 179
Query: 181 KVAGAMHDKSTQSNAHMALAMALVKHNGLIAEAYARSLVRSALQILDGDTAESSSQKRLS 240
V A+ S Q++ M L M+L KHN LI EAYAR L+ + L+IL +E +SQKRLS
Sbjct: 180 NVVVALDSNSNQTHLQMGLVMSLAKHNPLIVEAYARLLIHTGLRILGFGVSEGNSQKRLS 239
Query: 241 AIQMINFFMKFVDPRSISSELSKVVDIMEQCQNDRMPFVRGAAFEASQTAKSIAAQKGSR 300
A+QM+NF MK +DPRSI SE+ ++ ME+CQ+D+M +VRGAA+EA T+K IAA+ S+
Sbjct: 240 AVQMLNFLMKCLDPRSIYSEVELIIKEMERCQSDQMAYVRGAAYEAMMTSKRIAAELESK 299
Query: 301 HEVSTSPMVGSNFQRRREKSPCRSLWNAKGMAASPVQFQSPESHVVDSSIMCESTITESP 360
E + GSNF RR C S+ P SPES + S +S + SP
Sbjct: 300 MEKGCRSVTGSNFSRRN----CSSIV--------PDYSLSPESQTLGSFSGYDSPVESSP 347
Query: 361 VSIGQSSCNFDQNRRT-NRRLWSNDG----VDVSLKDGLFFRLCSDGKYLEDDLDEVCDS 415
+S +SCN + +RR+ NR+LW D VD+SLKDGLF R+ V DS
Sbjct: 348 IS--HTSCNSEFDRRSVNRKLWRRDENGGVVDISLKDGLFSRVTKGST-------TVSDS 398
Query: 416 EVTDTNFECTNS--FSGFVSASPNGATSRDKTPSPQASE-RPISIDDVKIYTTPRKLLRS 472
+ + C N F GF+ S R+ TPSPQ R I+ +D I++TPRKL+ S
Sbjct: 399 PLVPYD-TCENGDEFEGFLMES-----LRNTTPSPQRQRSRRINAEDFNIFSTPRKLISS 452
Query: 473 LQSSYDFD 480
LQ D D
Sbjct: 453 LQYPDDVD 460
>AT3G03970.1 | chr3:1028144-1029891 REVERSE LENGTH=555
Length = 554
Score = 280 bits (717), Expect = 1e-75, Method: Compositional matrix adjust.
Identities = 150/295 (50%), Positives = 198/295 (67%), Gaps = 4/295 (1%)
Query: 1 MGRSLSPLLRQELDNLDKDADSRRNAMKALKSYAKHLDSKSIPHFLAEVSDNKAAPGLPS 60
MGR+L RQEL NLDKD DS + AM L+S K LD+K + F+A++SD K GL S
Sbjct: 1 MGRNLGSAFRQELANLDKDPDSHKTAMSNLRSIVKDLDAKVVHVFVAQLSDVKEI-GLES 59
Query: 61 GEFTISLYEVLARVHGRNIVPQVGNIMSTIMCTLSSSGGSFPLHQACSKVVPAIARYGID 120
G +T+SL+E LAR HG I P + IM I+ TLSSS GS + QACS+ V A+ARYGID
Sbjct: 60 GGYTVSLFEDLARAHGVKIAPHIDIIMPAIIRTLSSSEGSLRVQQACSRAVAAMARYGID 119
Query: 121 PSTPDEEKAGIIASLCKPLCGALMG--NQDGAASGAALCLKALVESSNWRFAAGETVNEV 178
P+TP+++K +I SLCKPL +L+ +Q A G+ALCLK+LV+ NWR A+ E VN V
Sbjct: 120 PTTPEDKKTNVIHSLCKPLSDSLIDSQHQQHLALGSALCLKSLVDCDNWRSASSEMVNNV 179
Query: 179 CLKVAGAMHDKSTQSNAHMALAMALVKHNGLIAEAYARSLVRSALQILDGDTAESSSQKR 238
C +A A+ S+++ +HMAL MAL KHN EAYAR V+S L+ILD E SQKR
Sbjct: 180 CQSLAVALEATSSEAKSHMALVMALSKHNPFTVEAYARLFVKSGLRILDLGVVEGDSQKR 239
Query: 239 LSAIQMINFFMKFVDPRSISSELSKVVDIMEQCQNDRMPFVRGAAFEASQTAKSI 293
L AIQM+NF MK ++P+SISSEL + ME+ Q D+ +V+ AA E + A+ +
Sbjct: 240 LLAIQMLNFLMKNLNPKSISSELELIYQEMEKYQKDQH-YVKMAAHETMRQAERL 293
>AT2G07170.1 | chr2:2975565-2978692 FORWARD LENGTH=821
Length = 820
Score = 48.9 bits (115), Expect = 9e-06, Method: Compositional matrix adjust.
Identities = 39/147 (26%), Positives = 67/147 (45%), Gaps = 5/147 (3%)
Query: 19 DADSRRNAMKALKSYAKHLDSKSIPHFLAEVSDNKAAPGLPSGEFTISLYEVLARVHGRN 78
D D+ + + L+ +HL + FL+ + D + + I L LAR H
Sbjct: 40 DRDTYQRGVDELEKTVEHLAPDKVSCFLSCILDTDSEQKSAVRKECIRLMGTLARFHEGL 99
Query: 79 IVPQVGNIMSTIMCTLSSSGGSFPLHQACSKVVPAIARYGIDPSTPDEEKAGIIASLCKP 138
+ P +G ++S+I+ L + AC + + +A S +++ G+ SL KP
Sbjct: 100 VGPYLGKMVSSIVKRLKDPDSV--VRDACIETMGVLAS---KMSCYEDQNFGVFVSLVKP 154
Query: 139 LCGALMGNQDGAASGAALCLKALVESS 165
L A+ SGAALCL +++SS
Sbjct: 155 LFEAIGDQNKYVQSGAALCLARVIDSS 181
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.314 0.127 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 12,252,760
Number of extensions: 487685
Number of successful extensions: 1100
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 1094
Number of HSP's successfully gapped: 4
Length of query: 622
Length of database: 11,106,569
Length adjustment: 105
Effective length of query: 517
Effective length of database: 8,227,889
Effective search space: 4253818613
Effective search space used: 4253818613
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 115 (48.9 bits)