BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0526800 Os11g0526800|AK068214
         (409 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G58940.1  | chr3:21781002-21783213 REVERSE LENGTH=619           50   2e-06
AT5G03100.1  | chr5:726832-727980 FORWARD LENGTH=308               49   5e-06

>AT3G58940.1 | chr3:21781002-21783213 REVERSE LENGTH=619
          Length = 618

 Score = 50.1 bits (118), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 28/115 (24%), Positives = 58/115 (50%), Gaps = 5/115 (4%)

Query: 47  QLPEDILYHIHTLMPMQDAARAACASRCFLRSWRFYPRLNL-NVNTLRIDKRAPSNDKLT 105
            LPE++  HI + +P + AA  +  S+ +L  W+F   L++ + + L  ++     D++ 
Sbjct: 6   NLPEEVRCHILSFLPTKHAALTSVLSKSWLNLWKFETNLDIDDSDFLHPEEGKAERDEIR 65

Query: 106 IDFISRVDPIMLNHAGTGVKMFKLTTHPCFS-LHPSYVDRWLQIAFAPGIKEFEL 159
             F+  VD ++     + ++ F L    C + +HP +V+RW+      G+ +  L
Sbjct: 66  QSFVEFVDGVLALQGDSPIEKFSLK---CITGIHPDHVNRWICNVLQRGVSDLYL 117



>AT5G03100.1 | chr5:726832-727980 FORWARD LENGTH=308
          Length = 307

 Score = 48.9 bits (115), Expect = 5e-06,   Method: Compositional matrix adjust.
 Identities = 66/296 (22%), Positives = 116/296 (39%), Gaps = 50/296 (16%)

Query: 48  LPEDILYHIHTLMPMQDAARAACASRCFLRSWRFYPRLNLNVNTLRIDKRAPSNDKLTID 107
           LP++IL+HI    P + A R +  S+ +   W   P +++  N                 
Sbjct: 14  LPDEILHHILANTPTKLAIRTSVLSKRWKHVWYETPSISIVCN----------------- 56

Query: 108 FISRVDPIMLN-----HAGTGVKMFKLTTHPCFSLHPSYVDRWLQIAFAPGIKEFELEMT 162
              RVDP  LN     ++   +K F +T     ++    +D W+ +A +   +   L  T
Sbjct: 57  ---RVDPDSLNKTLSSYSTPKIKSFDVTISRDVTVPE--IDTWINLALSRKAENVSLRFT 111

Query: 163 RVSKMDYDFPCSLLSRVASSIQSFLLGGCSFHPGIQIGQMSTLTSLRLRSVKITEEELCG 222
                 Y F  +     +    S  L  C  +P   +   S+L +L L   K++++ +  
Sbjct: 112 S----HYRFRDTFFINSSLKQLSLTLVYCILNPKCVVS-WSSLRNLSLNRCKVSDDSIAK 166

Query: 223 FLSKSCALQRLLLSDCHNIVVLKIPHLLELNYLEVL------------HFRKLEVIDSSA 270
            L+    L+ L L+ C  +  L +   L L  LE+L            H R L + +   
Sbjct: 167 ILTGCSLLESLTLNLCDRLNDLDLSKSLSLRRLEILGDRWTPERIVAPHIRYLRLENYQR 226

Query: 271 PKLSTFIYAGPPIQISLGEALLQVKKMQMF-CDGSPDALHYGSKKLPSIAPNIQKL 325
           P  ST +      + +LG   L    +  F C+   ++L Y  ++      NI+KL
Sbjct: 227 P--STLVDVSSLTEANLG---LSKHVLDYFTCEMETESLQYMVRQTVVKLQNIKKL 277



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.138    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,786,587
Number of extensions: 352923
Number of successful extensions: 1051
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 1067
Number of HSP's successfully gapped: 3
Length of query: 409
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 308
Effective length of database: 8,337,553
Effective search space: 2567966324
Effective search space used: 2567966324
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 113 (48.1 bits)