BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0220300 Os11g0220300|AK068820
         (219 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G32520.1  | chr1:11758422-11759272 REVERSE LENGTH=240          141   3e-34

>AT1G32520.1 | chr1:11758422-11759272 REVERSE LENGTH=240
          Length = 239

 Score =  141 bits (355), Expect = 3e-34,   Method: Compositional matrix adjust.
 Identities = 79/147 (53%), Positives = 85/147 (57%), Gaps = 14/147 (9%)

Query: 30  RSLFSWGGRSA-----PPPEFQYHD-VAPPFPMSLVANTHLRGRELKCCYKATVDGFSAT 83
            SLF W          PP +  YHD V  PF +S+V  T L+GRELKCCYKA++DGF AT
Sbjct: 25  NSLFGWNSGKKIDNIRPPQQPAYHDDVEIPFSLSMVNKTFLKGRELKCCYKASIDGFGAT 84

Query: 84  DFHRRCDFKGPCXXXXXXXXXX-XXXXXSPEGYRSTDDYYDTLDAFLFYWXXXXXXXXXX 142
            FH RCDFKGPC                SPEGYRSTDDYYDT DAFLFYW          
Sbjct: 85  KFHERCDFKGPCVIIAYTKDKSFKFGGFSPEGYRSTDDYYDTFDAFLFYW-------LED 137

Query: 143 XXXXXXXXXXGGSGAALFDYARGGPQF 169
                     GGSGAALFDYARGGPQF
Sbjct: 138 CDDPIVLPKVGGSGAALFDYARGGPQF 164



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.139    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,445,689
Number of extensions: 165487
Number of successful extensions: 608
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 606
Number of HSP's successfully gapped: 1
Length of query: 219
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 124
Effective length of database: 8,502,049
Effective search space: 1054254076
Effective search space used: 1054254076
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 109 (46.6 bits)